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Updated: Jul 24, 2025

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化胺离子的碰撞诱导解离

Arnold Steckel¹, Dávid Papp^1,2, Katalin Uray³

¹MTA-ELTE Lendület Ion Mobility Mass Spectrometry Research Group, Department of Analytical Chemistry, ELTE Eötvös Loránd University, Budapest 1117, Hungary.

Journal of the American Society for Mass Spectrometry

|July 6, 2023

概括

负电子喷射电离 (ES-) 双重质谱 (MS/MS) 揭示了从素化中受益的HNCO损失,提供了补充的结构见解. 这项研究详细介绍了ES-中的HNCO损失机制,有助于区分素酸和脱酸序列.

科学领域:

蛋白质组学和分析化学
质谱测量质量谱测量
翻译后修改翻译后修改

背景情况:

阳性电喷电离 (ES+) 双重质谱法 (MS/MS) 是用于标识的标准.
负电喷射电离 (ES-) 提供了补充的结构信息,特别是在翻译后修改 (PTM) 时.
在ES-中素的碎片化以前没有被研究过.

研究的目的:

使用负电喷射电离 (ES-) MS/MS.探索素化的碎片化.
描述ES-中素化的HNCO损失,并提出一个反应机制.
为了将ES-碎片化模式与ES+进行比较,并确定素的诊断离子.

主要方法:

通过循序渐进的碰撞能量依赖测量,研究了9种素.
在QTOF和Q-Orbitrap仪器上利用了高分辨率,高精度的质谱学.
分析了碎片化模式,包括中性损失和序列离子形成.

主要成果:

观察到有利的,从前体和ES-中的碎片中选择性丧失HNCO,类似于ES+.
在ES-中,HNCO损失强度往往高于ES+.
确定了对Asp和Glu残留物的偏好裂解N端,以及来自序列离子的显著中性损失.

结论:

这项研究描述了ES-中素化的HNCO损失,并首次提出了一个机制.
在ES-MS/MS中,有利的HNCO损失和N端切割到Asp/Glu残留物可以区分氨酸和脱胺序列.
虽然ES-光谱需要人工检查,但这些发现增强了它在蛋白质组学中用于PTM分析的实用性.

关键词:

在CID CID中,你会发现.在MS/MS/MS上使用.素化是指素化.氨酸 (Citrulline) 是一种氨酸.脱阿米迪化脱阿米迪化无化酸是没有质的酸.消极模式消极模式

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