正常棒光受体突触的三维超结构和由视网膜脱落引起的退行性变化
Gil Torten1, Steven K Fisher2,3, Kenneth A Linberg4
1Departments of Ophthalmology and Neurobiology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095.
概括
高分辨率的3D成像揭示了杆光受体突触的复杂结构. 视网膜脱落会导致显著的超结构性变化,破坏视觉信息流.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 视觉科学 视觉科学 视觉科学
背景情况:
- 棒光受体突触对于暗光视觉至关重要,但其复杂的3D组织尚不清楚.
- 以前的方法导致了关于其组件精确排列的分歧.
研究的目的:
- 用电子显微镜断层扫描阐明哺乳动物棒突触的3D超结构.
- 详细介绍视网膜脱落后在杆突触中发生的结构变化.
主要方法:
- 电子显微镜 (EM) 断层扫描被用来生成猫视网膜棒突触的高分辨率3D体积.
- 分析集中在正常和实验性脱落的视网膜上.
主要成果:
- 突触带被分解为一个单一的结构,具有一个弧形密度,表明一个单一的释放点.
- 鉴定出一个四层次的 postsynaptic 过程 (两个水平细胞,两个棒双极细胞) 的排列.
- 视网膜脱落导致树的退缩,突触带的碎片化,以及横向细胞的telodendria的损失.
结论:
- 电磁断层扫描提供了对突触复杂组织的准确3D描述.
- 视网膜脱落会导致杆突触发生严重,破坏性的超结构变化,影响视觉信号传输.
相关概念视频
01:20Anatomy of the Eyeball
7.2K
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle...
7.2K
01:32The Retina
69.2K
The retina is a layer of nervous tissue at the back of the eye that transduces light into neural signals. This process, called phototransduction, is carried out by rod and cone photoreceptor cells in the back of the retina.
69.2K