关于混合物测试的DPRA的适用性:挑战和机遇
Quinten Marcelis1, Eric Deconinck2, Vera Rogiers3
1In Vitro Toxicology and Dermato-Cosmetology (IVTD), VUB, Laarbeeklaan 103, 1090, Jette, Belgium. quinten.marcelis@vub.be.
Archives of toxicology
|July 6, 2023
概括
直接反应性测定 (DPRA) 有效地评估已知混合物的皮肤敏感性. 然而,使用低于推的度可能会导致将强有力的敏感剂错误地归类为非敏感剂,从而影响其用于未知混合物的使用.
科学领域:
- 毒理学和化学安全
- 皮肤病学和皮肤健康
背景情况:
- 直接活性测定 (DPRA) 是一种在化学上验证的方法,用于评估蛋白质活性,这是皮肤敏感化的关键事件.
- 经合组织TG 442C指南允许DPRA用于多成分物质和已知的混合物,尽管关于其应用的数据有限.
研究的目的:
- 评估DPRA对单个物质和混合物的预测性能,特别是在偏离标准测试度时.
- 通过减少复杂性来评估DPRA对测试未知成分混合物的适用性.
主要方法:
- 实验A:评估DPRA对单个物质的预测能力,基于LLNA EC3值的度,而不是标准的100毫米.
- 实验B:对已知敏感剂,敏感剂与非敏感剂的混合物以及多种非敏感剂的混合物进行DPRA评估.
主要成果:
- 一种强有力的敏化剂 (oxazolone) 在0.4mM (其EC3度) 测试时被错误地归类为非敏化剂,而不是100mM.
- 对于二进制混合物,DPRA成功识别了敏感剂,最强的敏感剂决定了整体的耗.
- 该DPRA证明了对具有良好的特征的混合物的效率,但需要谨慎处理非标准度,特别是对于未知的混合物.
结论:
- 该DPRA是有效的评估皮肤敏感性在已知的,特征混合物.
- 偏离推的100毫米度,特别是强烈的敏化剂,可能导致错误的阴性结果,并限制未知混合物的适用性.
- 仔细考虑测试度对于准确的DPRA解释至关重要,特别是在复杂化学混合物的风险评估中.
更多相关视频
09:34Implementation of Portable Emissions Measurement Systems PEMS for the Real-driving Emissions RDE Regulation in Europe
Published on: December 4, 2016
28.2K
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
9.7K
相关概念视频
Non-destructive Tests for Concrete Strength
The rebound hammer test, also known as the Schmidt hammer test, is a non-destructive technique for evaluating the hardness of concrete and, indirectly, the strength of concrete. It operates on the principle that the rebound of a spring-driven mass from a concrete surface correlates to the surface's hardness. The device comprises a mass within a tubular housing, a spring mechanism, and a plunger that strikes the concrete. Upon release, the energy imparted to the mass by the spring causes it to...
Plasticizers
Water-reducers, or plasticizers, are chemical admixtures used in concrete to improve strength and workability. These additives reduce the water-cement ratio without compromising workability, lower the cement content while maintaining the same workability, or increase workability to assist concrete placement in inaccessible areas.
Plasticizers function by using surface-active agents to create repulsive electrostatic forces between cement particles. This dispersion enhances the concrete's...
Plasticizers function by using surface-active agents to create repulsive electrostatic forces between cement particles. This dispersion enhances the concrete's...
Toxicity Testing in Animals
Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
