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内部小核细胞RNAs通过基配对与它们相邻的内部序列调节宿主基因拼接

Danny Bergeron¹, Laurence Faucher-Giguère², Ann-Kathrin Emmerichs³

¹Département de Biochimie Et Génomique Fonctionnelle, Faculté de Médecine Et Des Sciences de La Santé, Université de Sherbrooke, Sherbrooke, Québec, J1E 4K8, Canada.

|July 6, 2023

概括

嵌入内部子的小核RNAs (snoRNAs) 可以调节它们的宿主基因.

科学领域:

分子生物学分子生物学
在RNA生物学,RNA生物学.
遗传学是一个遗传学.

背景情况:

小核RNAs (snoRNAs) 是非编码RNAs,主要参与rRNA成熟.
大多数哺乳动物的snoRNA都是内部的,通过宿主基因转录和拼接产生.
内部snoRNAs对宿主基因表达的调节作用在很大程度上是未知的.

研究的目的:

研究内部snoRNAs在调节宿主基因拼接中的潜在作用.
在拼接控制中探索snoRNA-宿主RNA相互作用的流行和机制.

主要方法:

人类RNA-RNA相互作用数据集的计算分析.
作为一个模型系统,研究了SNORD2-EIF4A2双层.
利用反感性寡核酸和突变来破坏snoRNA-intron结构.

主要成果:

30%的检测到的snoRNA与宿主转录相互作用,通常在替代拼接地点附近.
SNORD2的snoRNA与其宿主 (EIF4A2) 内子的相互作用阻碍了替代性外子的拼接.
破坏这种相互作用促进了替代的外子拼接,并改变了转录比率.

结论:

内部的snoRNAs可以作为它们宿主基因拼接的调节者.
靠近替代外因子的SnoRNA-宿主RNA复合体表明用于拼接控制的广泛机制.
这项研究支持内部snoRNAs在调节宿主转录成熟中的更广泛作用.

关键词:

替代拼接规则替代拼接规则互联网监管 (Cis-regulation) 是一种对互联网的监管.在Intron 里面.无稽之谈中介的衰变是没有意义的.RNA的二级结构是RNA的二级结构.RNA-RNA 相互作用这就是SnoRNA.斯诺RNA/宿主基因关系关系

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