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普遍的Sua5/TsaC家族为合成关键tRNA修饰的不同机制进化

Adeline Pichard-Kostuch¹, Violette Da Cunha¹, Jacques Oberto¹

¹CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Gif-sur-Yvette, France.

Frontiers in microbiology

|July 7, 2023

概括

在tRNA中对N6-threonyl-carbamoyl adenosine (t6A) 合成至关重要的TsaC/Sua5酶家族,显示Sua5是祖先形式. TsaC通过多个SUA5域损失进化,横向基因转移解释了其不整齐的分布.

科学领域:

进化生物学是进化的生物学.
分子生物学分子生物学
生物化学生物化学

背景情况:

TsaC/Sua5酶家族对于合成N6-threonyl-carbamoyl adenosine (t6A) 是必不可少的,这是对翻译精度至关重要的tRNA修改.
TsaC 蛋白质是单域的,而 Sua5 蛋白质既具有 TsaC 类域,又具有功能不明的额外 SUA5 域.
在t⁶A合成中,TsaC和Sua5的进化起源和独特机制尚未得到充分理解.

研究的目的:

研究TsaC和Sua5蛋白质的进化历史和分布.
了解TsaC从Sua5的出现以及域损失的功能影响.
探索水平基因转移和基因侵蚀在塑造这些酶的分布中的作用.

主要方法:

在多种生物体中对TsaC/Sua5蛋白家族进行基因分析.
进行比较序列和结构分析,以确定保存和分离的特征.
对基因分布模式的检查,包括共发生,特定血统的缺失和水平基因转移事件.

主要成果:

TsaC/Sua5家族无处不在,但Sua5似乎是祖先形式,TsaC源于多个SUA5域丢失事件.
义务共生体是唯一缺乏sua5或tsaC基因的生物,这表明功能冗余或基本性.
水平基因转移和域损失解释了TsaC和Sua5的当前不齐分布;TsaC的域损失导致了基质结合中的适应性突变.

结论:

Sua5是祖先的酶,TsaC通过SUA5域的反复损失以及适应性突变而演变.
在特定的考古群体中,TsaC/Sua5的分布是由基因丢失,横向基因转移和潜在的基因侵蚀形成的.
这项研究阐明了这些同源酶的进化轨迹,为未来关于t⁶A合成和翻译忠实性的功能研究提供了基础.

关键词:

在 Sua5 5 的位置.美国 TsaC TsaC 酵素酶是一种酶.演化演化演化演化演化演化演化 t6A 一个很好的.这是一个tRNARNA.普遍的蛋白质是一种普遍的蛋白质.

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