CD7蛋白在瘤中的T细胞透中起着至关重要的作用
Binjie Sheng1,2, Kailu Zhang1, Shuaiyu Tian1
1Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China.
Heliyon
|July 7, 2023
概括
CD7蛋白对于T细胞透到瘤中至关重要,而不是为造血系统的发展. CD7淘汰赛小鼠显示CD8+T细胞瘤透受损,瘤生长增加.
科学领域:
- 免疫学 免疫学 免疫学
- 血液学 血液学 血液学
- 在瘤学瘤学.
背景情况:
- CD7蛋白是CD7+淋巴瘤的治疗点.
- CD7在造血系统中的作用尚不清楚.
研究的目的:
- 研究CD7在造血系统发育中的功能和T细胞介导的抗瘤免疫.
- 评估CD7淘汰赛 (KO) 对免疫细胞群和瘤进展的影响.
主要方法:
- 产生了CD7淘汰赛 (KO) 的小鼠,并将它们与野生类型 (WT) 的 littermates进行了比较.
- 评估了骨髓中的造血分化,以及胸腺和脏中的细胞群.
- 用B16-F10黑色素瘤细胞注射免疫小鼠来评估瘤生长.
- 进行了体外检测,以分析CD8+T细胞迁移和粘附.
- 在正常的T细胞和各种瘤细胞系中阻断了CD7功能 (Jurkat,CCRF-CEM,KG-1a).
主要成果:
- CD7 KO并没有影响胆小板和脏中的造血细胞分化或免疫细胞组成.
- 在CD7 KO小鼠中,瘤生长得更快.
- 在脏和瘤中CD8+ T细胞的比例在CD7 KO小鼠中降低.
- 在体外,CD8+ T细胞透和粘附在CD7 KO小鼠中受损.
- 阻止CD7减少了Jurkat,CCRF-CEM和KG-1a细胞系的迁移和入侵,但不是正常的T细胞.
结论:
- CD7对造血系统发育不至关重要.
- CD7在促进T细胞透到瘤中起着至关重要的作用.
- 向CD7可能通过影响T细胞瘤透影响抗瘤免疫力.
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