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Circ_0102543通过miR-942-5p/SGTB轴抑制肝细胞癌的进展

Shiming Yang¹, Dianye Wang², Rui Zhang³

¹Department of General Surgery Shanxi Provincial People's Hospital Taiyuan China.

Annals of gastroenterological surgery

|July 7, 2023

概括

循环RNAcirc_0102543通过调节microRNA-942-5p/SGTB轴来抑制肝细胞癌 (HCC) 的进展. 这种circRNA/miRNA/蛋白质通路为HCC治疗提供了潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

肝细胞癌 (HCC) 是一个重要的全球健康问题.
循环RNAs (circRNAs) 越来越多地被认可为它们在癌症发展中的作用,包括HCC.
在HCC瘤发生过程中,circ_0102543的特定功能需要进行详细的研究.

研究的目的:

调查circ_0102543在肝细胞癌 (HCC) 进展中的作用.
为了阐明涉及circ_0102543,microRNA-942-5p (miR-942-5p) 和HCC细胞中的SGTB的调节机制.
评估circ_0102543/miR-942-5p/SGTB轴在HCC中的治疗潜力.

主要方法:

定量实时PCR (qRT-PCR) 用于测量circ_0102543,miR-942-5p和SGTB的表达水平.
在体外测试包括MTT,EDU,transwell和流细胞计,以评估HCC细胞行为.
西方斑点分析检查蛋白质表达水平并确认调节机制.

主要成果:

在Circ_0102543和SGTB表达下调,而miR-942-5p在HCC组织上调.
Circ_0102543作为miR-942-5p的分子海绵,SGTB被确定为miR-942-5p的目标.
过度表达circ_0102543抑制了HCC细胞的增殖,迁移和入侵在体外和体内.

结论:

通过调节miR-942-5p/SGTB轴,Circ_0102543可以抑制HCC的进展.
该circ_0102543/miR-942-5p/SGTB监管网络在HCC瘤发生中发挥着至关重要的作用.
这一轴代表了肝细胞癌的一个有前途的潜在治疗点.

关键词:

我们的SGTB是SGTB.环_010254343 的时间表.肝细胞癌是肝细胞癌.在 miR-942-5p 中.

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