用不透的冷保护剂和子水扩展器进行狗精子玻璃化
Anton Antonov1, Boyana Ivanova1
1Department of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.
Animal reproduction
|July 7, 2023
概括
子水扩展剂含有大豆莱西丁和糖糖有效地玻璃化狗精子,保持精子运动性和形态学,用于临床应用.
科学领域:
- 生殖生物学 生殖生物学
- 低温生物学 低温生物学
- 兽医科学 兽医科学 兽医科学
背景情况:
- 狗精液冷保存对于遗传保存和人工授精至关重要.
- 开发具有高精子存活率的简单有效的玻璃化方法对于临床使用至关重要.
研究的目的:
- 为了评估一种以子水为基础的扩展剂的有效性,并补充了大豆莱丁和糖糖,用于犬种子玻璃化.
- 评估狗精子的解后生命力,运动性和形态.
主要方法:
- 从成年狗中收集精液,然后在子水扩展器中与大豆莱西丁 (1%) 和糖糖 (0.25M) 稀释.
- 精液使用直接入液的方法进行了玻璃化.
- 解后的评估包括活力,运动参数和精子形态.
主要成果:
- 玻璃化导致活性的精子百分比,正常形态和运动率下降 (P<0.05).
- 玻璃化后的关键速度参数 (VCL,VSL,VAP,LIN,ALH,BCF) 没有受到影响 (P>0.05).
- 该方法显示了对常规犬精子冷保存的高潜力.
结论:
- 子水扩展剂含有大豆莱西丁和糖是狗精液玻璃化的一个有前途的冷保护剂.
- 这种简单的方法提供了一种可行的选择,用于临床应用的保存犬精子.
- 进一步的研究可以优化协议,以提高精子存活率.
相关概念视频
01:22Sperm Structure and Semen Composition
2.9K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
2.9K
01:15Sperm Transport
959
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
959