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感染SARS-CoV-2诱导HMGB1通过转化后修饰和PANoptosis的分泌

Man Sup Kwak^1,2, Seoyeon Choi^1,3, Jiseon Kim^3,4

¹Department of Microbiology, Yonsei University College of Medicine, Seoul 03722, Korea.

|July 7, 2023

概括

严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 感染触发高流动性组盒1 (HMGB1) 通过主动和被动途径释放. 一种新的细胞死亡形式PANoptosis与SARS-CoV-2感染期间的HMGB1被动释放有关.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
病毒学病毒学

背景情况:

严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 感染导致严重的炎症和器官损伤.
高流动性组盒1 (HMGB1),一种与损伤相关的分子模式,与炎症性疾病有关.
HMGB1分泌与促炎性刺激和病毒感染有关.

研究的目的:

为了研究SARS-CoV-2感染期间HMGB1分泌的机制.
确定是否涉及主动和被动释放通路.
探索 PANoptosis 在 HMGB1 释放中的作用.

主要方法:

使用HEK293E/ACE2-C-GFP和Calu-3细胞系进行体外研究.
分析了HMGB1.1的翻译后修改 (乙化,酸化,氧化).
在人类和小鼠的肺组织中使用免疫组织化学和免疫光学.

主要成果:

通过翻译后的修改,SARS-CoV-2感染诱导了HMGB1的活性分泌.
证明PANoptosis是SARS-CoV-2感染期间HMGB1被动释放的新途径.
在受感染的人类和小鼠肺组织中确认了HMGB1的转移和释放.

结论:

HMGB1分泌是SARS-CoV-2病变发生的一个关键事件.
活跃的HMGB1释放涉及特定的翻译后修改.
泛光死在被动HMGB1释放中发挥关键作用,导致SARS-CoV-2引起的炎症和损伤.

关键词:

这是一种HMGB1蛋白质.这就是PANoptosis.后翻译修改后的修改.严重急性呼吸系统综合征冠状病毒2

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