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在Trueperella pyogenes中发现了新型的化物-林科萨米德-斜谱蛋白B抗性基因 (ERM) (56)

Emma Marchionatti^1,2, Vincent Perreten¹

¹Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern , Bern, Switzerland.

|July 7, 2023

概括

在Trueperella pyogenes中发现了一种新的抗生素耐药性基因, erm(56),来自于一只狗. 这种基因使得格拉姆阳性和格拉姆阴性细菌对宏类,林科萨米德和链条蛋白B抗生素产生抗性.

科学领域:

兽医微生物学兽医微生物学
抗微生物耐药性抗微生物耐药性
分子生物学分子生物学

背景情况:

特鲁佩雷拉 (Trueperella pyogenes) 是一种重要的动物病原体.
抗菌素耐药性是一个日益严重的全球健康问题.
麦克罗利德,林科萨米德和链条蛋白B (MLS_B) 耐药性往往是由核糖体RNA甲基酶基因介导的.

研究的目的:

在Trueperella pyogenes中识别新的抗菌素耐药性机制.
描述一个新发现的耐药性基因的功能和遗传背景.

主要方法:

一个耐药的Trueperella pyogenes分离物的全基因组测序.
在T. pyogenes和Escherichia coli中克隆和表达基因.
对基因侧边区域的生物信息分析和数据库搜索.

主要成果:

一个新的23S核糖体RNA甲基酶基因, erm(56),被确定.
erm56) 基因在T. pyogenes和E. coli中赋予了MLS_B耐药性.
在移动遗传元素 (IS6100,1级整体) 和来自牲畜的其他细菌物种中发现了erm56) 基因.

结论:

erm(56) 基因代表了MLS_B耐药性的新机制.
在移动元件和各种细菌上发现的erm(56) 表明它已经广泛传播,并有可能进一步传播.
在动物中使用抗生素可能会推动这种新型耐药基因的选择和传播.

关键词:

在DNA测序过程中,DNA测序基因表达的基因表达方式麦克罗利德-林科萨米德-链谱图 B 阻力机制的阻力机制.

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