核心保存的转录性调节网络定义了侵入性多细胞细胞系
Ha T H Vu1,2, Regan L Scott3, Khursheed Iqbal3
1Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA.
Development (Cambridge, England)
|July 7, 2023
概括
研究人员比较了老鼠和人类的侵入性热囊细胞,发现保存的基因调控网络对于胎盘发育至关重要. 这项研究增强了对在血位移中的子宫 - 胎盘界面的理解.
科学领域:
- 生殖生物学和发育科学.
- 比较的基因组学和表观基因组学.
- 哺乳动物的胎盘研究.
背景情况:
- 鼠是研究血位移的有价值模型,因为它们与人类的侵入性热囊细胞功能有相似之处.
- 关于规范跨物种的侵袭性多细胞细胞种群的保护性调节机制的知识有限.
- 了解这些机制对于破译胎盘发育和功能至关重要.
研究的目的:
- 为了研究和比较老鼠和人类入侵性热囊细胞的染色质可访问性概况.
- 识别保护的基因调节网络,控制侵袭性多细胞细胞系.
- 为了进一步了解在血位移中的子宫 - 胎盘接口.
主要方法:
- 产生和整合单核ATAC-seq和单细胞RNA-seq数据从大鼠不同妊娠阶段的子宫 - 胎盘接口组织.
- 确定各种细胞类型的染色质可访问性概况,包括侵入性热细胞.
- 在老鼠的侵入性热囊细胞和人类的外流性热囊细胞之间对染色质可访问性的比较分析.
主要成果:
- 在大鼠模型中确定了侵入性热囊细胞,自然杀手细胞,巨细胞,内皮细胞和光滑肌细胞的独特染色体可访问性概况.
- 在老鼠和人类侵入性热囊细胞之间的可访问的染色质区域中发现了基因调节的保存模式和丰富的动机.
- 发现了一种保存的基因调节网络,对侵袭性热囊细胞功能至关重要.
结论:
- 这项研究揭示了大鼠和人类侵入性热囊细胞之间基因调节机制的显著相似之处.
- 已经确定了一个保存的基因调节网络,为这种细胞系提供了对这种细胞系的基本调节机制的洞察.
- 这些发现支持大鼠作为动物模型的实用性,并为未来对胎盘发育的研究提供了基础.
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