紫外线通过调节METTL3促进黑色素的产生.
Haoran Guo1, Hongliang Zeng2, Yibo Hu1
1Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, China.
Journal of cellular physiology
|July 7, 2023
概括
紫外线 (UV) 辐射通过N6-甲基氨酸 (m6 A) 修饰促进皮肤色素的产生. 这种由METTL3调节的表观遗传过程增加了YAP1的表达,促进了黑色素的产生.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
背景情况:
- 紫外线 (UV) 辐射是导致皮肤颜色变化的主要外部因素.
- 紫外线诱导的黑色素发生的精确机制,特别是表观遗传调节,需要进一步阐明.
- N6-甲基氨酸 (m6 A) 修饰是影响各种生物过程的关键表观遗传机制.
研究的目的:
- 调查m6的作用和机制 紫外线辐射 (UVB) 诱导的黑色素发生的修饰.
- 探索METTL3,YAP1和UVB照射下的黑色素生成之间的关系.
主要方法:
- 在UVB照射后对黑色素细胞和MNT1细胞的全球m6A水平的分析.
- 对METTL3和与黑色素发生有关的基因的基因表达分析.
- 在MNT1细胞中METTL3的过度表达和敲除.
- 评估YAP1蛋白水平和m6 A修饰部位.
- 抑制YAP1的表达,以评估其在METTL3诱导的黑色素发生中的作用.
主要成果:
- 低剂量UVB增加了全球m6在黑色素细胞和MNT1细胞中的修饰.
- 在阳光照射皮肤中,METTL3表达与MITF正相关.
- 过度表达METTL3可提高黑色素含量和黑色素生成基因的调节,而降低能降低这些基因的调节,这种效应被UVB放大.
- METTL3调节的YAP1蛋白水平,YAP1mRNA上的三个预测的m6A位点得到证实.
- 抑制YAP1部分逆转了METTL3诱导的黑色素发生.
结论:
- 紫外线B辐射增强了全球m6 在黑色素细胞的修改.
- METTL3由UVB上调调节,并通过通过m6 A修改增加YAP1表达来促进黑色素的产生.
- 这一途径涉及METTL3,YAP1和TEAD1,在UVB诱导的黑色素发生过程中起着重要作用.
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