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多参数表面等离子体共振仪器用于多核酸定量检测.

Huixiang Wang¹, Honggang Wang¹, Yafeng Huang¹

¹College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.

Biomedical microdevices

|July 7, 2023

概括

此摘要是机器生成的。

这项研究引入了一种快速,无标签的表面等离子体共振 (SPR) 仪器,用于多重核酸检测. 这种新型系统能够快速准确地识别像miRNA-21和miRNA-141这样的生物标志物,这对于癌症诊断至关重要.

关键词:

生物传感器是一种生物传感器.多个核酸多个核酸.核酸杂交的核酸杂交方式表面等离子体共振 (SPR) 是一种

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科学领域:

生物医学工程生物医学工程
分子诊断学分子诊断
生物传感技术的技术

背景情况:

多重核酸测定对于疾病诊断,环境监测和食品安全至关重要.
传统方法面临的局限性包括复杂的操作,长检测时间,不稳定的标签和信号干扰.
需要快速,灵敏和用户友好的多重核酸检测平台.

研究的目的:

开发一个实时,快速,无标签的表面等离子体共振 (SPR) 仪器,用于多重核酸检测.
解决复杂生物样本多重检测和定量分析的挑战.
为DNA和miRNA等小分子提供简单有效的临床检测 (POCT) 平台.

主要方法:

开发一个使用总内反射,线性光源,镜,光探测器和机械传输的多参数光学系统.
实施适应性值一致性校正算法,以管理频道变化并实现定量比较.
使用表面等离子体共振 (SPR) 来无标签和无放大检测目标核酸.

主要成果:

在30分钟内,SPR仪器成功地实现了miRNA-21和miRNA-141的无标签和无放大检测.
该系统表现出良好的重复性和特异性,目标寡核酸的检测极限 (LOD) 为50nM.
最小可检测的绝对样品量约为4 pmol,突出显示了高灵敏度.

结论:

开发的SPR仪器为多重核酸检测提供了一个简单,高效和快速的解决方案.
这项技术有可能作为各种小分子 (包括癌症生物标志物) 的临床检测 (POCT) 平台.
无标签和无放大方法克服了传统核酸分析的关键局限性.