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鉴定和实验验证帕金森病与重度抑郁症的相关性常见基因

Huiqing Wang¹, Shanshan Dou², Chunmei Wang³

¹School of Medicine, Southeast University, Nanjing, Jiangsu, 210009, People's Republic of China.

Molecular neurobiology

|July 7, 2023

概括

帕金森病 (PD) 和严重抑郁症 (MDD) 具有共同的遗传联系. 该研究确定了AQP9,SPI1和RPH3A作为参与PD和MDD共发生的关键基因,突出了免疫细胞的作用.

科学领域:

神经科学是一个神经科学.
遗传学是一个遗传学.
免疫学免疫学免疫学

背景情况:

帕金森病 (PD) 是一种流行的神经退行性疾病,影响全球数百万人.
重度抑郁症 (MDD) 是PD的一个常见,但经常被忽视的非运动症状.
连接PD和MDD的潜在分子机制仍然不完全理解.

研究的目的:

确定候选基因和阐明与PD和MDD的同时发生相关的分子途径.
探索PD和MDD之间共享的差异表达基因 (DEG) 的功能作用.
验证关键基因并调查免疫细胞在患有MDD的PD中的参与.

主要方法:

从基因表达总汇 (GEO) 下载并分析了PD (GSE6613) 和MDD (GSE98793) 的基因表达概况.
确定了具有一致表达趋势的常见DEG,其次是基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
构建了蛋白质-蛋白质相互作用 (PPI) 网络,利用LASSO回归来识别关键基因,并使用独立数据集 (GSE99039,GSE201332) 和qPCR验证了发现.

主要成果:

确定了45种常见的DEGs,富含中性粒细胞脱粒化和白细胞激活等途径.
通过LASSO回归和网络分析选择了AQP9,SPI1和RPH3A作为关键验证基因.
在PD和MDD模型中确认了AQP9,SPI1和RPH3A的高表达.
在PD中观察到中性粒细胞和单细胞的失调,这表明它们在PD和MDD病变发生过程中的作用.

结论:

基因AQP9,SPI1和RPH3A与PD和MDD的共享病理生理学有关.
免疫细胞的透,特别是中性粒细胞和单细胞,在PD和MDD的发展中起着重要作用.
这些发现为PD和MDD同时发生的机制提供了新的见解.

关键词:

生物信息学是一种生物信息学.免疫系统的免疫过程.大型抑郁症主要是抑郁症.作为一个中性粒细胞.帕金森病是帕金森氏症的一种疾病.

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