在口腔状细胞癌中Rac1激活作为与淋巴结转移相关的预测因素
Masae Yamazaki1, Shoichi Sekikawa2, Taiki Suzuki3,2
1Department of Oral Oncology, Oral and Maxillofacial Surgery, Tokyo Dental College, Chiyoda, Japan. t.yamazakimasae@gmail.com.
International journal of clinical oncology
|July 7, 2023
概括
与Ras相关的C3毒素基质1 (Rac1) 是舌头状细胞癌转移的关键因素. 高Rac1表达预测了二次淋巴结转移,为早期疾病提供了潜在的生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 病理学 病理学 病理学
背景情况:
- 二次淋巴结转移 (SLNM) 是早期舌状细胞癌 (TSCC) 的关键预后因素.
- 由于缺乏统一的标准,预测SLNM仍然具有挑战性.
- 与Ras相关的C3毒素基质1 (Rac1) 涉及表皮-介质细胞过渡 (EMT) 和癌症进展,成为潜在的治疗标.
研究的目的:
- 调查Rac1在TSCC早期转移中的作用.
- 确定Rac1表达与临床病理特征之间的关联,包括SLNM.
- 评估Rac1作为TSCC中SLNM的潜在预测因子.
主要方法:
- 免疫组织化学染色被用于评估Rac1表达在69个I/II阶段TSCC标本.
- 分析了Rac1水平与包括SLNM在内的临床病理特征之间的关联.
- 在体外实验中,在口腔状细胞癌 (OSCC) 细胞系中进行了Rac1沉默的实验,以检查其功能作用.
主要成果:
- 高Rac1表达显著与侵入深度增加,瘤芽,血管入侵和SLNM相关 (p <0.05).
- 单变异分析确定了Rac1表达,入侵深度和瘤芽作为SLNM显著预测因素 (p <0.05).
- 多变量分析显示,Rac1表达是SLNM的唯一独立决定因素. 在实验室中Rac1下调显示出细胞迁移和增殖减少的趋势.
结论:
- Rac1在口腔状细胞癌的转移过程中发挥着重要作用.
- 在早期的TSCC中,Rac1表达是二级淋巴结转移的有价值的独立预测器.
- 向Rac1可能为管理TSCC转移提供一种新的治疗策略.
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