相关实验视频

Updated: Jul 24, 2025

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

从养殖场养的鱼类中分离出来的产生生物膜的葡萄球菌中多药耐药的表型:分子审查

Muneeb Hamza^1,2, Gopalan Krishnan Sivaraman², Mukteswar Prasad Mothadaka³

¹Research Scholar, Faculty of Science, Cochin University of Science and Technology, Cochin 682022India.

Journal of applied microbiology

|July 7, 2023

概括

在养殖场养的鱼类中发现了耐甲基金色葡萄球菌 (MRSA),它们表现出多种药物耐药性,并携带毒性基因. 这凸显了水产养殖业加强生物安全的必要性,以防止抗菌素耐药性传播.

科学领域:

微生物学微生物学
水产养殖是水产养殖的一种方式.
抗微生物耐药性抗微生物耐药性

背景情况:

水产养殖是一个不断增长的粮食来源,引发了人们对潜在的病原体传播的担忧.
甲素耐药黄金葡萄球菌 (MRSA) 是一个重要的公共卫生问题.
养殖鱼类中MRSA的存在和特征需要进行调查.

研究的目的:

为了确定从喀拉拉州水产养殖场的鱼类中甲素耐药葡萄球菌 (MRS) 的流行率.
描述MRSA分离物的分子特征,包括抗微生物耐药性和毒性因子.
了解MRSA克隆在这种环境中的遗传多样性.

主要方法:

从180个鱼样中分离和识别了耐甲林的葡萄球菌.
针对β-乳酸和诺基诺的抗微生物敏感性测试.
检测病毒性基因 (sea, seb, seg, sei) 和生物膜相关基因 (icaA, icaD).
使用spa类型和序列类型 (STs) 的测序进行分子类型化.

主要成果:

45个MRS分离体 (25%) 被恢复,其中73.33%被确定为多药耐药 (MDR).
发现病毒性基因 (sea, seb, seg, sei) 与抗菌素耐药性基因 (ARG) 存在并存.
MRSA分离物 (n=17) 属于三个spa类型 (t345,t2526,t1309) 和三个序列类型 (ST772,ST88,ST672).

结论:

养殖场养殖的鱼类含有各种MRSA菌株,具有显著的抗菌素耐药性和毒性潜力.
这些发现强调了分子表征对于有效的控制策略的重要性.
预防措施对于限制水产养殖系统内抗微生物耐药性病原体的传播至关重要.

关键词:

孟加拉湾的克隆人在MLST上,我们可以看到MLST.水产养殖的水产养殖生物膜生产商的生产者养殖场养殖的鱼是养殖场养殖的鱼.这是一个spa-typing.

更多相关视频

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay

Published on: May 5, 2016

相关概念视频

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Biofilms

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...