谷氨进口系统满足金黄色葡萄球菌 (Staphylococcus aureus) 营养硫的要求,并促进物种间的竞争
Joshua M Lensmire1, Michael R Wischer1, Cristina Kraemer-Zimpel1
1Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America.
PLoS genetics
|July 7, 2023
概括
金色葡萄球菌利用一种新的谷氨进口系统 (gisABCD) 来从减少和氧化谷氨中获取必需的硫. 这个系统提供了对抗Staphylococcus epidermidis的竞争优势.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 营养素的获取 营养素的获得
背景情况:
- 硫对于细菌生长至关重要.
- 黄金葡萄球菌使用谷氨 (GSH) 作为硫,但吸收机制尚不清楚.
研究的目的:
- 识别和描述Staphylococcus aureus从谷氨中获得营养硫的机制.
- 研究这种系统在物种间竞争中的作用.
主要方法:
- 基因位置识别 (gisABCD操作子).
- 用GSH/GSSG进行细菌繁殖的表型分析.
- 酶活性测定γ-氨基甲酸转化酶 (Ggt).
- 同类系统的生物信息分析.
主要成果:
- 一个五基因的基因位点,gisABCD,使S. aureus能够使用GSH或GSSG作为唯一的硫源生长.
- 编码的Ggt酶从GSH和GSSG中释放谷氨酸,位于细胞质中.
- 在相关的葡萄球菌中存在同类系统,但在S. epidermidis中不存在.
- 在GisABCD-Ggt系统赋予S. aureus比S. epidermidis一个竞争优势.
结论:
- 在S. aureus中发现了一种新的硫获取系统 (GisABCD-Ggt),该系统针对GSH和GSSG.
- 这种系统提高了S. aureus在人类微生物群中的其他葡萄球菌的竞争力.
- 细胞质中Ggt的局部化很少见,黄金色细菌是第二个例子.
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