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通过操纵微流体基于纸张的芯片上的咖啡环效应,实现双模式不扭曲的视觉光传感战略
Ji Qi1,2, Bowei Li1, Dong Lin1
1CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
Analytical chemistry
|July 7, 2023
概括
本研究引入了一种双模式策略,以防止使用智能手机的微流体纸质分析设备 (μPAD) 的光图像扭曲. 该方法提高了检测尿液中的histidine的准确性,改善了视觉光传感能力.
科学领域:
- 分析化学 分析化学
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
背景情况:
- 基于智能手机的光传感提供了方便,但遭受了图像扭曲.
- 基于纸张的微流体分析设备 (μPAD) 需要灵敏而准确的检测方法.
- 现有的方法由于光学工件而难以进行定量分析.
研究的目的:
- 开发一种新的双模式策略,用于在μPAD上进行未扭曲的视觉光传感.
- 为了提高基于智能手机的光检测的灵敏度和准确性.
- 为了快速和方便地分析人类尿液中的histidine.
主要方法:
- 操纵咖啡环效应以控制μPAD上的液体样本行为.
- 开发一种双模式分析策略,包括RGB像素分析和光条长度测量.
- 使用牛血清蛋白稳定金纳米集群-铜离子复合体作为光探针.
- 采用智能手机和一个小型成像盒来获取数据.
主要成果:
- 实现了对视觉光传感器进行改进的反扭曲.
- 通过将水平方向分成600个像素进行光图像的定量分析.
- 确定了0.021毫米的RGB分析和0.5毫米的条形长度测量检测极限 (LOD).
- 在人类尿液中证明了对histidine的快速和方便的测试.
结论:
- 这种新的双模式策略有效地克服了智能手机引起的光图像在μPADs上的扭曲.
- 这种方法显著提高了视觉光传感的定量准确性和灵敏度.
- 开发的方法显示了对点诊断和现场应用的巨大潜力.
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