用于测定血清乳酸脱酶活性的光度流量系统
Justyna Głowacka1, Agnieszka Wiśniewska2, Robert Koncki1
1University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093, Warsaw, Poland.
Talanta
|July 7, 2023
概括
本研究提出了两种改进的方法,用于使用新型流量分析系统来确定乳酸脱酶 (LDH) 活性. Fe-ferrozine方法为血清样本提供了更高的选择性和更低的检测极限.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 常规的乳酸脱酶 (LDH) 活性测定在近紫外线测量方面面临挑战,特别是对于血清样本.
- 标准方法依赖于监测NADH度,这在复杂的生物矩阵中可能是有问题的.
研究的目的:
- 开发和比较两个修改后的LDH活性测试,利用NADH的降解性质.
- 为这些测试实施和评估一个完全机械化的多通换流分析配对发射体检测二极管 (MCFA-PEDD) 系统.
- 评估开发的方法的分析性能和适用于真实人血清样本分析的适用性.
主要方法:
- 开发了两种测试修改:一种使用铁的铁离子还原,另一种使用蓝 (NBT) 还原.
- 完全机械化的MCFA-PEDD系统采用电磁体设备,用于两种测定方法.
- 测试性能根据线性范围,检测极限和在常见干扰物质存在时的选择性进行了评估.
主要成果:
- 在Fe-ferrozine和NBT方法中,分别实现了6.0-200.0 U L-1和10.0-250.0 U L-1的线性范围.
- 确定Fe-ferrozine的检测极限为0.2 U L-1和NBT的4.5 U L-1,低量化极限允许样本稀释.
- 与NBT方法相比,Fe-ferrozine方法对LDH活性具有更高的选择性,特别是在葡萄糖,酸,白蛋白,白素,铜和离子的存在下.
结论:
- 开发的MCFA-PEDD系统有效地支持这两种修改后的LDH测定方法.
- Fe-ferrozine方法是比NBT方法更有选择性和敏感的替代方法,用于在人血清中确定LDH活性.
- 统计分析证实了开发的方法与参考方法之间的满意一致性,突出了它们的分析实用性.
更多相关视频
06:18Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Published on: June 21, 2018
11.5K
09:34The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
Published on: July 27, 2018
18.2K
相关概念视频
01:14UV–Vis Spectrometers
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
01:26Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.