在垂体腺瘤中 lncRNAs 的表达模式
Soudeh Ghafouri-Fard1, Maryam Khaledabadi2, Ghazal Najafi3
1Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran.
Pathology, research and practice
|July 7, 2023
概括
这项研究发现,四种长非编码RNAs (lncRNAs) 在非功能性垂体腺瘤 (NFPAs) 中过度表达. 这些lncRNAs可能在NFPA的发展和进展中发挥作用,其中一些具有作为诊断标记物的潜力.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 非功能性垂体腺瘤 (NFPA) 缺乏激素过量的临床表现.
- 在NFPA开发的基础上的分子机制尚未完全理解.
- 长非编码RNAs (lncRNAs) 正在成为各种癌症的关键调节者.
研究的目的:
- 研究NFPA中五种特定的lncRNAs (FGD5-AS1,ATP6V0E2-AS1,ARHGAP5-AS1,WWC2-AS2和EPB41L4A-AS1) 的表达水平.
- 探索这些lncRNAs作为NFPA诊断和预后生物标志物的潜力.
- 为了确定临床参数和NFPA特征之间的相关性.
主要方法:
- 使用定量实时PCR来评估NFPA组织和相邻的非瘤组织中的lncRNA表达.
- 使用统计分析,包括t测试和千平方测试,来评估表达差异和临床关联.
- 进行曲线下面面积 (AUC) 分析,以评估lncRNAs的诊断潜力.
主要成果:
- 与非瘤样本相比,ATP6V0E2-AS1,EPB41L4A-AS1,FGD5-AS1和WWC2-AS2在NFPA中的表达显著增加 (P <0.05).
- 在NFPA和非瘤样本之间,ARHGAP5-AS1表达没有显著差异 (P = 0.62).
- EPB41L4A-AS1和FGD5-AS1显示了区分NFPA和非瘤组织的潜力,尽管AUC值不是最佳的.
- 瘤的侵入性与患者的年龄和瘤大小 (P <0.05) 有积极的关联.
- 疾病持续时间与脑脊髓液 (CSF) 泄漏相关 (P = 0.023).
- 瘤大小与Knosp分类和侵入性相关 (P < 0.05).
结论:
- 该研究发现NFPAs中特定lncRNAs的显著失调.
- 作为NFPA的潜在诊断生物标志物,EPB41L4A-AS1和FGD5-AS1需要进一步调查.
- 年龄,瘤大小和疾病持续时间等临床因素与NFPA的侵入性和特征有关.
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