相关实验视频

Updated: Jul 24, 2025

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Published on: March 6, 2018

双和 perfluoroalkyls 改变人类干细胞的完整性:与不孕不育的可能联系

Giulia Gaggi¹, Andrea Di Credico¹, Federica Barbagallo²

¹Reprogramming and Cell Differentiation Lab, Center for Advanced Studies and Technology (CAST), 66100, Chieti, Italy; Department of Medicine and Aging Sciences, "G. D'Annunzio" , University of Chieti-Pescara, 66100, Chieti, Italy; UdA -TechLab, "G. D'Annunzio", University of Chieti-Pescara, 66100, Chieti, Italy.

Environmental research

|July 7, 2023

概括

产前暴露于内分泌干扰物,如双和 perfluoroalkyls 损害人类的干细胞. 这些化学物质,包括BPA和PFOS,影响早期发育,并可能影响未来的生育能力.

科学领域:

毒理学毒理学毒理学
发育生物学发展生物学
干细胞生物学干细胞生物学

背景情况:

双和 perfluoroalkyls 是工业化学品被认为是内分泌干扰剂 (EDs).
消化不良药可以模仿激素,在摄入后导致各种疾病.
产前暴露是令人担忧的,因为这些化学物质穿过胎盘屏障,并在发育中的胚胎中积累.

研究的目的:

研究双-A (BPA),双-S (BPS), perfluorooctane-sulfonate (PFOS) 和 perfluorooctanoic-acid (PFOA) 单独或组合对人类诱导的多能干细胞 (hiPSC) 的影响.
评估这些EDS对与多能性,生殖线特异性和表观遗传调节相关的基因的影响.

主要方法:

利用人类诱导的多能干细胞 (hiPSCs) 作为模型系统,与胚胎细胞干细胞共享特征.
暴露在单个和组合ED的hiPSC:BPA,BPS,PFOS和PFOA.
分析了对细胞活力 (甲基毒性) 和基因表达模式的影响.

主要成果:

暴露于这些EDs诱导了hiPSCs显著的线粒毒性.
观察到对维持多能性,生殖系特异性和表观遗传调节至关重要的基因发生了剧烈的变化.
联合暴露显示了添加剂,协同作用和负面影响,其结果不容易预测.

结论:

产前接触这些EDs可能会损害胚胎干细胞的完整性.
对关键早期人类发育阶段的干扰可能会影响未来的生育能力.
综合性ED暴露的不可预测影响强调需要更好地意识到其复杂的健康影响.

关键词:

双醇是一种双醇.通过DNA甲基化.内分泌干扰物干扰内分泌干扰物生殖线规范细菌线的规格.人类诱导的多能干细胞 perfluoroalkyls 的使用情况

更多相关视频

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Published on: April 26, 2018

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

相关概念视频

Teratogenicity

Teratogenicity

The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...