植物染色体相互作用因子PIF1对烟草中干旱耐受性和胡卜素生物合成产生负面调节
Shaohua Liu1, Yinchao Zhang2, Xuhao Pan2
1Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, China; Shenzhen Yupeng Technology Co., Ltd, Shenzhen 518110, China.
International journal of biological macromolecules
|July 7, 2023
概括
植物染色体相互作用因子 (PIF) 在植物生理学中发挥作用. 在烟草中,NtPIF1负面调节干旱耐受性和胡卜素生物合成,为开发抗压作物提供了潜力.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 农业科学 农业科学
背景情况:
- 植物染色体相互作用因子 (PIF) 是调节植物生长和发育的关键转录因子.
- 一些PIF的特定作用,包括烟草 (Nicotiana tabacum L.) 中的NtPIF1,尚未完全理解,特别是在应激反应中.
研究的目的:
- 在烟草中克隆和描述PIF转录因子NtPIF1.
- 研究NtPIF1在干旱压力反应和胡卜素生物合成中的作用.
主要方法:
- 克隆和特征的NtPIF1.1.
- 用CRISPR/Cas9基因编辑进行NtPIF1淘汰和过度表达研究.
- 生理学测试以评估干旱耐受性 (奥斯摩斯调整,抗氧化活性,光合作用,水损失).
- 基因表达分析和分子测试 (电泳运动移动转移,双化酶) 来确定基因调节.
主要成果:
- 在干旱压力下,NtPIF1转录水平增加,并局部化到核中.
- NtPIF1淘汰赛提高了干旱耐受性,而过度表达导致了敏感性.
- NtPIF1通过直接结合关键基因 (NtNCED3,NtABI5,NtZDS,Ntβ-LCY) 的促进子区域来抑制酸 (ABA) 和胡卜素生物合成.
结论:
- NtPIF1作为干旱应激适应和烟草中胡卜素生物合成的负调节剂.
- 使用CRISPR/Cas9对NtPIF1进行有针对性的淘汰,提高了对干旱的耐受性,这表明它有可能开发抗压作物品种.
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