ресвератрол可以保护HepG2细胞免受脱氧尼瓦诺诱导的铁亡
Pengju Wang1, Qing Yao1, Dan Zhu1
1Medicine Research Institute, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, PR China.
Toxicology
|July 7, 2023
概括
脱氧尼瓦伦醇 (DON) 通过铁亡导致肝细胞死亡. 复星 (Res) 通过通过SLC7A11-GSH-GPX4通路抑制铁化,从而防止DON的毒性.
科学领域:
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 脱氧尼瓦伦醇 (DON) 是一种普遍存在的真菌毒素,导致肝细胞死亡.
- DON诱导的肝毒性机制,特别是新的细胞死亡途径,需要进一步阐明.
- 铁,一种独特的依赖铁的细胞死亡形式,与各种毒性有关.
研究的目的:
- 为了研究铁化在脱氧尼瓦伦醇 (DON) 诱导的HepG2细胞毒性的作用.
- 探索白醇 (Res) 对DON诱导的铁亡的保护作用.
- 阐明了Res对DON毒性的抗性作用背后的分子机制.
主要方法:
- 用DON和/或Res对待HepG2细胞.
- 评估了细胞活力,增殖,与铁死相关的基因表达 (GPX4,SLC7A11,Nrf2),脂质过氧化 (MDA,4-HNE),活性氧物种 (ROS) 和Fe (II) 水平.
主要成果:
- DON暴露导致GPX4,SLC7A11和Nrf2的表达减少,Fe (II) 过载增加,脂质过氧化和ROS产生,诱导铁亡.
- 复星预治疗逆转了DON诱导的变化,减弱了铁亡,并改善了细胞活力和增殖.
- 复星通过激活SLC7A11-GSH-GPX4信号通路来表现出抗费洛的作用.
结论:
- 复星可以改善HepG2细胞中Deoxynivalenol诱导的铁亡.
- 这项研究确定了铁亡作为DON肝毒性的关键机制.
- Resveratrol显示出作为治疗剂对抗DON诱导的肝损伤的潜力.
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