高效的mlo基粉状菌耐药性在六类小麦中,没有类效应
Christina R Ingvardsen1, Julio A Massange-Sánchez2, Finn Borum3
1Department of Agroecology, AU-Flakkebjerg, Aarhus University, DK-4200 Slagelse, Denmark.
概括
在小麦中使用Mlo基因突变引入粉状菌抵抗是有效的. 结合三种Mlo基因的突变,可以提供持久的耐药性,同时可以控制负面副作用.
科学领域:
- 植物育种 植物育种
- 遗传学 是一个遗传学.
- 农作物保护 农作物保护
背景情况:
- 基于Mlo的耐药性是大麦中耐久粉状菌耐药性的成功策略.
- 这种来自Mlo基因突变的抵抗机制在各种植物物种中都存在.
- 将基于mlo的耐药性引入到六倍体小麦中是具有挑战性的,因为有三个同源的Mlo基因 (Mlo-A1,Mlo-B1,Mlo-D1).
研究的目的:
- 为了在六小麦中引入持久的mlo-based抗粉状菌的耐药性.
- 调查同源Mlo基因突变对疾病耐药性和类型症状的影响.
- 为了确定在小麦中有效抵抗粉状菌的最佳突变策略.
主要方法:
- 对由乙基甲硫酸盐 (EMS) 生成的突变小麦植物进行Mlo-A1,Mlo-B1和Mlo-D1.1突变的选.
- 在三个同源的Mlo基因中选择和组合多个突变,以创建三重同源的突变系.
- 在现场条件下对突变系对粉状菌的耐药性的评估,并评估类效应.
主要成果:
- 在实地试验中,24个三重同卵性mlo突变小麦品种表现出对粉状菌的高度有效抵抗力.
- 所有18种已识别的突变都导致了耐药性,但对性斑点和性斑点等性症状的影响有所不同.
- 不同的突变组合影响了不良类效应的严重程度.
结论:
- 突变所有三种Mlo同类物质对于在小麦中实现高效的粉状菌耐药性至关重要.
- 纳入至少一个较弱的突变可以帮助减轻与基于mlo的耐药性相关的强烈类效应.
- 这一战略平衡了强有力的疾病控制与小麦育种中最小化的有害副作用.
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