环境丰富增加了前额叶皮质中的Bdnf转录:对表观遗传控制机制的含义
Gabriel Araújo Costa1, Nívea Karla de Gusmão Taveiros Silva1, Priscila Marianno1
1Pharmacology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Neuroscience
|July 7, 2023
概括
环境丰富提高了特定的大脑衍生神经营养因子 (BDNF) 异子表达的调节,并减少了小鼠前额叶皮层中的DNA甲基化. 这表明BDNF外子表达的表观遗传控制,特别是涉及外子IV,由于环境丰富.
科学领域:
- 神经科学是一个神经科学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 行为科学 行为科学
背景情况:
- 环境丰富 (EE) 调节大脑生理和行为,可能通过脑衍生神经营养因子 (BDNF).
- 在EE下,特定的Bdnf外因表达和表观遗传调节的理解仍然很差.
研究的目的:
- 研究54天EE暴露在小鼠前额叶皮层 (PFC) 中对BDNF的转录和表观遗传效应.
- 为了分析个别的BDNF外显子mRNA表达和关键调节器的DNA甲基化,外显子IV.
主要方法:
- 在54天EE暴露后,对C57BL/6雄性小鼠 (n=33) 的PFC中Bdnf异构II,IV,VI和IXmRNA表达的分析.
- 在 Bdnf exon IV 的两个 CpG 位点评估 DNA 甲基化概况.
- 评估类似焦虑的行为和血皮质水平.
主要成果:
- 在PFC中,EE上调了Bdnf外因子II,IV,VI和IX的mRNA表达.
- 在 Bdnf exon IV 的两个 CpG 位点,EE 降低了 DNA 甲基化水平.
- 没有观察到类似焦虑的行为或皮质激素水平的显著变化.
结论:
- 暴露于EE会诱导PFC的表观遗传修饰,特别是减少Bdnf外IV的DNA甲基化.
- 研究结果表明,EE诱导BDNF外因子表达的表观遗传控制,可能涉及外因子IV甲基化.
- 这项研究阐明了在环境丰富下在PFC内Bdnf基因的转录和表观遗传调节.
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