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涉及Bacillus subtilis natto产生的水溶性复合物menaquinone-7中的K结合因子的表征

Toshiyuki Chatake¹, Yasuhide Yanagisawa², Risa Murakami²

¹Insititute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori, Asashironishi-2, Osaka 590-0484, Japan.

Journal of biochemistry

|July 7, 2023

概括

细菌素 natto产生一种水溶性维生素K2 (menaquinone-7) 和的复合物,称为K结合因子 (KBF). 这项研究揭示了KBF.

科学领域:

生物化学生物化学
分子生物学分子生物学
营养科学营养科学

背景情况:

维生素K,特别是menaquinone-7 (MK-7),由于其高生物可用性,对骨和心血管健康至关重要.
由于其水溶性差,MK-7的治疗应用受到限制.
细菌素 natto产生含有MK-7和的水溶性复合物,称为K结合因子 (KBF).

研究的目的:

阐明Bacillus subtilis natto.产生的K结合因子 (KBF) 的结构特征.
了解KBF如何促进menaquinone-7的水溶性.

主要方法:

用质谱法分析KBF的分子量和成分.
聚烯胺凝电泳 (PAGE) 用于估计KBF的分子量.
氨基酸分析确定了KBF的组成氨基酸.
逆相高性能液态染色学 (RP-HPLC) 用于分离.

主要成果:

质谱学表明在m/z = 1050时有显著的峰值,与之前的PAGE估计为KBF的~3kDa形成鲜明对比.
氨基酸分析显示,KBF由由9种氨基酸组成的1kDa组成,其中Asx,Glx,Val,Leu和Met是最丰富的.
这些具有类似洗剂的特性,可以通过RP-HPLC分离出来.
一个小细胞结构是由三个~1kDa的捆绑形成的,在内部封装了menaquinone-7.

结论:

KBF的基本单元是一个具有洗剂特性的~1kDa.
这些1kDa中的3个组装成一个3kDa捆.
这一捆形成一种水溶性小胞结构,有效地溶解了menaquinone-7.

关键词:

细菌细菌纳托 natto.K-结合因子的结合因子这种药物是menaquinone-7-7 溶解化溶解化溶解化维生素K. 简称:CV,柱体体积;DLS,动态光散射;FPLC,快速蛋白质液体染色学;KBF,K结合因子;MK-7,menaquinone-7; natto-MK-7,menaquinone-7的水溶性复合物;RH,水力动态半径.

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