从谷物中选具有抗原作用和潜在益生菌性质的乳酸细菌
Min Ju Seo1, Sung-Min Won1, Min Ju Kwon1
1Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Scientific reports
|July 7, 2023
概括
从谷物中分离出来的乳酸细菌显示出作为益生菌的潜力. 三种菌株,Pediococcus pentosaceus K28,Levilactobacillus brevis RP21和Lactiplantibacillus plantarum RP12,有效地抑制了脂肪生成,并表现出益生菌的特性.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 营养科学 营养科学
背景情况:
- 乳酸细菌 (LAB) 越来越多地被认为具有健康益处.
- 谷物衍生微生物是新型益生菌菌株的潜在来源.
- 脂肪生成,即脂肪细胞形成的过程,是代谢健康干预的关键目标.
研究的目的:
- 从韩国谷物中分离和识别乳酸细菌.
- 评估选择的LAB菌株的抗增脂效应和益生菌潜力.
- 调查有希望的LAB候选人的安全性和功能性质.
主要方法:
- 隔离和16S rRNA基因测序用于细菌识别.
- 随机放大多态DNA (RAPD) -PCR用于菌株分化.
- 在体外检测细胞毒性,脂肪生成抑制,基因表达分析 (PPARγ,C/EBPα,CD36,LPL,FAS,ACC),酸/胆盐耐受性,Caco-2细胞粘附性和抗生素耐药性.
- 用于酶活性评估的API ZYM套件.
主要成果:
- 在187个隔离物中选择了25种代表性的LAB菌株.
- 佩迪奥科克托萨塞乌斯K28,莱维拉克托巴西勒斯布雷维斯RP21,拉克提普兰蒂巴西勒斯植物RP12显著抑制了C3H10T1/2细胞中的脂质积累,没有细胞毒性.
- 这三种菌株下调了关键的脂肪基因标记基因,并表现出有利的益生菌特征,包括酸/胆盐耐受性和Caco-2细胞粘附性.
结论:
- 精选的谷物衍生LAB菌株,特别是P. pentosaceus K28,L. brevis RP21和L. plantarum RP12,具有显著的抗脂原性质.
- 这些菌株表现出强大的益生菌潜力,包括在恶劣条件下生存和良好的粘附能力.
- 已识别的LAB菌株需要进一步调查,作为代谢健康应用的功能成分.
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