Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Spatial transcriptome for cell segmentation.

Nature computational science·2026

Same author

Correction to "Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA".

Analytical chemistry·2026

Same author

Seminal Fluid Biomarkers for Early Cancer Detection: A Systematic Review.

Biomedicines·2026

Same author

Thigmotactic navigation and functional enrichment of boar sperm in microfluidic channels.

Reproduction (Cambridge, England)·2026

Same author

Endocrine therapy reprogramming of breast cancer facilitates metastatic escape via upregulation of P-Rex1/Rac1 signalling.

Nature communications·2026

Same author

Lattice and ligand engineering for hierarchical heterogeneous nanocrystals.

Proceedings of the National Academy of Sciences of the United States of America·2026

Search research articles

相关实验视频

Updated: Jul 24, 2025

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

一种用于快速定量评估微流体学中的粒子分类,使用基本细胞计设备的方法.

Robert Salomon^1,2, Sajad Razavi Bazaz^1,2,3, Wenyan Li²

¹Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

|July 8, 2023

概括

本研究介绍了一种流细胞计法,用于准确测量微流体粒子分离. 这种技术可以量化单个细胞和集群的性能,即使在复杂的混合物中.

关键词:

细胞分类细胞分类细胞计量细胞计量方法微流体学在微流体学方面分离式隔离器的使用方法

更多相关视频

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

相关实验视频

Last Updated: Jul 24, 2025

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

科学领域:

生物技术是生物技术.
分析化学分析化学
微流体学微流体学

背景情况:

目前用于评估微流体分离装置的方法在准确性和适用于复杂样品的限制.
高速成像和手动细胞计数对于精确的性能特征是不够的,特别是高度或细胞群.

研究的目的:

介绍和详细介绍一种使用流细胞计的新方法,用于对连续流微流体颗粒分离装置的定量性能评估.
通过使复杂的高度混合物能够进行准确的评估,克服现有方法的局限性.

主要方法:

使用流细胞计与脉冲处理量化分析粒子分离.
应用该方法来评估单细胞和细胞群,例如循环瘤细胞 (CTC) 群.
整合细胞表面表型化,对复杂细胞混合物的详细分析.

主要成果:

开发的流细胞计法准确量化了分离效率和样品纯度.
它成功地评估了复杂混合物中单个细胞和细胞的设备性能.
与传统技术 (如血细胞计或细胞计数器) 相比,该方法显示出更高的能力.

结论:

这种基于流量细胞计的方法为评估微流体分离装置提供了强大而准确的方法.
它可以准确地描述用于分离单细胞和生物相关集群的设备.
该方法将加速先进的微流体分离技术的开发和验证.