微流体双层平台研究共同培养的神经网络与单向突触连接之间的功能相互作用
Yana Pigareva1,2, Arseniy Gladkov1,2, Vladimir Kolpakov1,2
1Neurotechnology Department, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950, Russia.
Micromachines
|July 8, 2023
概括
这项研究引入了一种新的微流体芯片,用于培养相互连接的神经网络. 该芯片可以检查一个网络中的活动如何影响另一个网络,从而提高我们对大脑连接的理解.
科学领域:
- 神经科学是一个神经科学.
- 生物工程是生物工程.
- 系统生物学 系统生物学
背景情况:
- 神经网络连接对于大脑功能和认知至关重要.
- 在体内异质网络中研究活动传播存在重大挑战.
研究的目的:
- 开发和验证一种新的双层PDMS微流体芯片,用于检查两个相互连接的神经网络之间的功能相互作用.
- 为了研究一个神经网络的活动对另一个神经网络活动的影响,具有单向突触连接.
主要方法:
- 在一个与微电极阵列集成的两室微流体芯片中利用海马神经元培养.
- 为单向轴突生长和网络连接设计了不对称的微通道.
- 应用了四毒素 (TTX),CPP和CNQX来局部调节神经元活动.
主要成果:
- 对源网络的局部四极毒素 (TTX) 应用没有影响目标网络的升率.
- 目标网络活动在TTX后1-3小时保持稳定,证明了局部化学调制的可行性.
- 在源网络中抑制突触活动改变了目标网络活动的时空特征.
结论:
- 新型的微流体芯片有效地促进了相互连接的神经网络之间的功能相互作用的研究.
- 证明了局部化学活动调制和异质神经元电路之间的电活动的影响.
- 为深入研究神经科学研究中网络层面相互作用提供了一个有价值的平台.
相关概念视频
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