通过Rynchophorus ferrugineus (Coleoptera: Curculionidae) 中的两个气味结合蛋白来识别宿主植物
Ying Huang1,2, Wei Hu1,2, You-Ming Hou1,2
1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China.
Pest management science
|July 8, 2023
概括
研究人员在红棕虫 (RPW) 中发现了两种臭味结合蛋白 (OBP),它们对于检测宿主植物挥发物和聚合激素至关重要,为新的害虫控制策略铺平了道路.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 化学生态化学生态学
背景情况:
- 红棕虫 (Rhynchophorus ferrugineus,RPW) 是棕种的一种重要害虫.
- 气味结合蛋白 (OBPs) 对于昆虫的嗅觉至关重要,并代表了害虫控制的潜在目标.
研究的目的:
- 调查两个特定的OBP,RferOBP8和RferOBP11在红棕虫的嗅觉感知中的作用.
- 探索这些OBP作为开发新型害虫控制策略的目标的潜力.
主要方法:
- 对RferOBP8和RferOBP11.11的组织表达特征和性二态的分析.
- 气色谱-质谱 (GC-MS) 用于分析宿主植物的挥发性物质.
- 分子对接和光竞争性结合试验,以评估OBP-干相互作用.
- 行为试验和RNA干扰 (RNAi) 来评估OBPs的功能意义.
主要成果:
- RferOBP8和RferOBP11在天线中高度表达,并表现出性二态.
- 这些OBP与八种棕挥发物和ferrugineol结合,包括聚合费洛蒙,具有很高的亲和力.
- 暴露于这些挥发性物质会引起成年RPW的吸引力,而减少OBP表达会损害行为反应.
结论:
- RferOBP8和RferOBP11是红棕虫对宿主挥发物和聚合激素的反应不可或缺的组成部分,影响寻找宿主的行为.
- 这些发现为开发用于RPW管理的创新行为干扰策略提供了基础.
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