尿路微生物组之间的差异,状腺硬化诱导和非状腺硬化诱导的尿道狭窄疾病
Marcus L Jamil1, Aaron Perecman1, Amanda Sherman1
1Lahey Hospital and Medical Center, 41 Burlington Mall Road, Burlington, MA, 01805, USA.
World journal of urology
|July 8, 2023
概括
在状硬化 (LS) 尿道狭窄疾病 (USD) 中观察到尿道微生物群差异. 在手术前的尿样显示,与非LS USD对照组相比,LS USD患者的多样性有显著的变化.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 微生物组研究的研究.
- 病原基因组学 病原基因组学 病原基因组学
背景情况:
- 状硬化 (LS) 是一种慢性炎症性皮肤疾病,可以影响生殖泌尿道.
- 尿道狭窄疾病 (USD) 是LS的常见并发症,导致尿道阻塞.
- 尿路微生物组在LS-USD的发病和进展中的作用尚不清楚.
研究的目的:
- 为了研究和比较患有LS-USD和非LS-USD的患者之间的尿道微生物组合.
- 评估尿路微生物组的术前和术后差异.
- 为了确定与LS-USD相关的潜在微生物生物标志物.
主要方法:
- 一项前性队列研究,包括为美元进行手术修复的患者.
- 采集和分析手术前和手术后的尿样.
- 细菌DNA提取,16S rRNA测序,以及微生物多样性和丰富性的生物信息分析.
- 在LS-USD和非LS-USD组之间的微生物特征的统计比较.
主要成果:
- 总共分析了69个尿样 (36个手术前,33个手术后).
- 在LS-USD和非LS-USD患者之间的手术前尿样中发现了α多样性的显著差异 (p=0.01).
- 贝塔多样性分析显示,基于疾病和手术状态的微生物社区结构存在显著差异 (p<0.002).
结论:
- 与非LS-USD对照组相比,患有LS-USD的患者表现出明显的尿路微生物组变化.
- 这些微生物差异主要在手术前明显.
- 需要进一步的研究来探索尿路微生物组在LS-USD病变发生,疾病严重程度和复发中的作用.
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