费洛蒙受体的进化转变有助于四种Helicoverpa物种的物种化
Song Cao1,2, Chen Shi3, Bing Wang1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Cellular and molecular life sciences : CMLS
|July 8, 2023
概括
研究人员确定了蝶激素受体 (ORs) 中的关键氨基酸,这些受体决定了特定物种的伴侣识别. 这项研究揭示了驱动昆虫交流和物种化进化的分子机制.
科学领域:
- * 分子生物学 * 分子生物学
- * 进化遗传学的进化遗传学
- * 化学生态学 化学生态学
背景情况:
- *雄使用特定的激素来识别伴侣,这对于繁殖隔离和物种化至关重要.
- * 了解费洛蒙受体 (OR) 的进化是研究密切相关物种的分歧的关键.
- * * Helicoverpa * 属为研究这些进化过程提供了一个模型.
研究的目的:
- * 为了确定四种*Helicoverpa*物种两种正体激素受体 (OR14b和OR16) 的功能差异化.
- *阐明这些OR的基质反应特异性背后的分子机制.
- * 开发一种综合方法来识别OR中负责激素选择性的关键残留物.
主要方法:
- *使用AlphaFold2结构预测的全原子分子动力学模拟.
- * 分子对接以预测参与基质结合的关键氨基酸残留物.
- *位点定向的突变发生和功能分析,以验证预测的残留物.
主要成果:
- * 在OR14b的正方体中,在164和232位的两个疏水性氨基酸决定了Z9-14:Ald和Z9-16:Ald的特异性.
- *一个单一的氨基酸在OR16的正方位66的位置决定了Z11-16:OH的特定结合,可能是通过全相互作用.
- *已确定特定的氨基酸残留物与费洛蒙基质直接相互作用.
结论:
- *开发了一种有效的综合方法,以确定OR基质选择性的关键残留物.
- *阐明了在蝶中激素识别系统多样化的分子基础.
- *这些发现有助于理解通过嗅觉受体进化驱动物种的分子机制.
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