Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

<i>Lactobacillus plantarum</i> ameliorates high-fat-diet-induced dyslipidemia <i>via</i> cholesterol metabolism and gut microbiota modulation.

Food & function·2026
Same author

AI-Guided Design and Predictive Modeling of Synthetic <i>Escherichia coli</i> Promoters through Comprehensive -10/-35 Box Engineering.

ACS synthetic biology·2026
Same author

Deep learning guided programmable design of Escherichia coli core promoters from sequence architecture to strength control.

Nucleic acids research·2025
Same author

Developing PhoR-PhoB-Based Biosensor by Directed Evolution for Application in Ultralow Inorganic Phosphorus Detection.

ACS sensors·2025
Same author

Engineering Xylose Metabolism and Coutilization with Glucose in <i>Corynebacterium glutamicum</i> for Efficient Microbial Cell Factories.

Journal of agricultural and food chemistry·2025
Same author

Metabolic Engineering of <i>Escherichia coli</i> for the Improved Malonic Acid Production.

ACS synthetic biology·2025
Same journal

Perfusion development and its potential for cell therapy manufacturing with adherent cells.

Applied microbiology and biotechnology·2026
Same journal

Bacterial degradation of aromatic ester pollutants in agro-ecosystems: implications for bioremediation.

Applied microbiology and biotechnology·2026
Same journal

Inhibiting biofilm growth on ammonium salt-functionalized or fluorinated voice prostheses silicone.

Applied microbiology and biotechnology·2026
Same journal

Enhancing reproducibility in mixing time determination of stirred tank reactors via automated analysis and standardized inter-laboratory trials.

Applied microbiology and biotechnology·2026
Same journal

Hybrid genome assembly and phenotypic assays reveal carbohydrate metabolism diversity in Lacticaseibacillus strains.

Applied microbiology and biotechnology·2026
Same journal

Ammonium and nitrate fluxes drive metabolic functional shifts in tap water biofilms.

Applied microbiology and biotechnology·2026
查看所有相关文章

相关实验视频

Updated: Jul 24, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.8K

基于生物传感器的高通量选使得有效的酸生产成为可能.

Rui Zhi1,2, Nan Cheng1,2, Guohui Li3,4

  • 1National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Applied microbiology and biotechnology
|July 8, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了一个生物传感器和选平台,以改善生物基酸生产,克服遗传局限性. 这导致了5L发酵器中的3.62g/L标位,推动了可持续的化学制造.

关键词:
亚酸是亚酸的一种.生物传感器是一种生物传感器.遗传的异质性 遗传的异质性高通量选的高通量选反向脂肪酸降解途径的逆向.

更多相关视频

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

相关实验视频

Last Updated: Jul 24, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.8K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.0K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

科学领域:

  • 生物技术是生物技术.
  • 代谢工程是代谢工程.
  • 合成生物学 合成生物学

背景情况:

  • 工业酸合成带来了环境污染的风险.
  • 生物基生产提供了一个可持续的替代品,但面临着影响产品产量的遗传异质性带来的挑战.
  • 提高菌株性能对于生物基化学品的工业化至关重要.

研究的目的:

  • 克服遗传异质性限制生物基酸生产.
  • 开发一个高通量选平台,用于识别高性能菌株.
  • 通过代谢工程和发酵优化来增强脂肪酸标位.

主要方法:

  • 反向脂肪酸降解途径的组成表达.
  • 一个酸生物传感器的设计和优化.
  • 建立一个使用生物传感器的高通量选平台.
  • 发酵优化和扩大规模的研究.

主要成果:

  • 选了一种高性能菌株,初始酸标位达到188.08 mg·L-1.1.
  • 优化发酵导致摇瓶中的标位为531.88 mg·L-1 (18.78倍的改善).
  • 在5L发酵器中进行大规模发酵,产生3.62g·L-1的脂肪酸.

结论:

  • 开发的策略有效地减少了微生物菌株的遗传异质性.
  • 高通量选平台是工业菌株开发的宝贵工具.
  • 这项工作为更高效的酸工业生物生产提供了基础.