RBOHF通过调节Arabidopsis中的反应性氧物种和可塑性pH动态来激活口腔免疫
Dominique Arnaud1, Michael J Deeks1, Nicholas Smirnoff1
1Biosciences, Faculty of Health and Life Sciences, University of Exeter, Exeter, EX4 4QD, UK.
The Plant journal : for cell and molecular biology
|July 8, 2023
概括
植物免疫反应依赖于口腔关闭,由活性氧物种 (ROS) 调节. 这项研究揭示了RBOHF在控制ROS信号传递中的关键作用,以有效地防御对细菌病原体的口腔防御.
科学领域:
- 植物免疫力 植物免疫力
- 植物生理学 植物生理学
- 蜂信号传输是如何进行的
背景情况:
- 胃关闭是植物对抗病原体入侵的关键防御机制.
- 无塑性反应性氧物种 (ROS),特别是过氧化 (H2O2),对于在细菌感知时启动口腔关闭至关重要.
- 在口腔防御期间,护卫细胞中细胞内ROS动态的精确调节仍然不清楚.
研究的目的:
- 为了研究NADPH氧化酶在调节细胞溶液H2O2签名时在口腔免疫反应中的作用.
- 阐明RBOHF和RBOHD在PAMP触发的口腔关闭和ROS生产中的特定功能.
- 了解护卫细胞中细胞质和细胞质ROS信号之间的相互作用.
主要方法:
- 在NADPH氧化酶 (rbohF,rbohD) 中使用了Arabidopsis thaliana突变.
- 使用H2O2传感器roGFP2-Orp1和基于光素的探针来监测ROS动态.
- 评估了对病原体相关分子模式 (PAMPs) 和外源H2O2.2的口腔关闭反应.
主要成果:
- 该rbohF突变体表现出改变的细胞质H2O2氧化,但损害了PAMP触发的口腔关闭和ROS生产.
- RBOHF,而不是RBOHD,对于PAMP介导的ROS爆发和护卫细胞中的细胞质化是必不可少的.
- 在较低度下,rbohF突变显示出H2O2介导的口腔关闭缺陷,而高的H2O2在野生型植物中没有诱导关闭.
结论:
- 在植物免疫反应期间,RBOHF在调节无形成性ROS产生和随后的口腔关闭方面发挥着至关重要的作用.
- 这项研究突出了护卫细胞中细胞质和细胞质ROS信号通路之间的复杂相互作用.
- 对于对抗细菌病原体的有效口腔防御,RBOHF是不可或缺的.
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