代谢和调节的整合揭示了快速适应在非本地基质上生长的适应性
Vikas D Trivedi1, Sean F Sullivan1, Debika Choudhury1
1Department of Chemical & Biological Engineering, Tufts University, Medford, MA, USA.
Cell chemical biology
|July 8, 2023
概括
工程酵母利用植物糖需要协调细胞和工程目标. 上游基因活动,而不是复杂的工程,限制了糖的使用,以改善生物基生产.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 有效的生物基生产依赖于工程微生物来进行基质价值化.
- 尽管进行了广泛的研究,但在Saccharomyces cerevisiae中利用半纤维素粉素仍然是一个重大挑战.
研究的目的:
- 确定限制酵母酵母糖利用量的关键因素.
- 开发一种简单而有效的策略来增强酸发酵.
主要方法:
- 实施一种半合成的 regulon 来控制基因表达.
- 代谢流量和生长动力学的分析.
- 非本地代谢基因与本地酵母调节系统的整合.
主要成果:
- 通过将蜂和工程目标与半合成调节剂协调,显著提高了生长率和产量.
- 控制糖流入中央碳代谢的上游基因被确定为限制速率的基因.
- 酵母新陈代谢表现出固有的适应性,能够在非本地基质上快速生长.
结论:
- 一种简约的,整体的方法,将非本地基因与本地基因结合在一起,为工程酸利用提供了一个有效的策略.
- 复杂系统的代谢工程并不总是实现高性能所必需的.
- 了解和优化上游代谢途径对于高效的基质价值化至关重要.
相关概念视频
Regulation of Metabolism
9.5K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
9.5K
Introduction to Metabolism
125
Metabolism encompasses all biochemical reactions in a living organism, facilitating both the breakdown and synthesis of biomolecules. These metabolic processes are categorized into catabolic and anabolic pathways, which operate in a coordinated manner to ensure energy balance and cellular function.Catabolic Pathways and Energy ReleaseCatabolic pathways involve the breakdown of complex macromolecules such as carbohydrates, lipids, and proteins into smaller structures like monosaccharides, fatty...
125
Metabolism of Chemolithotrophs
52
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
52
Overview of Metabolism
31.3K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
31.3K
Operon Model
40
The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
40
Other Glycolytic Pathways
49
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
49


