识别诱导亡的活性氧物种,这是一种新和独特的受调细胞死亡模式

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Updated: Jul 24, 2025

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

Ko Eto¹, Takuya Suemoto¹

¹Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Japan.

Experimental cell research

|July 8, 2023

概括

反应性氧物种 (ROS),特别是超氧化物 (O2-) 和过氧化 (H2O2),共同诱导细胞亡,一种新的细胞死亡. 亚酸和联合处理模仿非热气压等离子体效应.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
血医学是一种血医学.

背景情况:

非热大气压等离子体 (NTAPP) 诱导一种独特的细胞死亡,称为亡.
具体的活性氧物种 (ROS) 和触发亡的机制以前是未知的.

研究的目的:

为了确定负责诱导亡的ROS类型.
阐明ROS引发亡的机制.
为了研究亚酸 (AA) 和抗菌素A (AM) 作为ROS生成剂用于亡.

主要方法:

用不同剂量的酸 (AA),抗菌素A (AM) 和过氧化 (H2O2) 治疗细胞.
评估细胞死亡标志物,包括细胞缩,Pdcd4消失,囊泡形成,DNA消化和膜透性.
使用抗氧化剂来确认ROS调解.
化学诱导的亡与NTAPP诱导的亡的比较.

主要成果:

高剂量的AA (产生O2-和H2O2) 和AM (产生O2-) 诱导细胞死亡与收缩,Pdcd4损失和囊泡.
单独的AA治疗导致了不规则的DNA消化和增加了膜的透性.
单独的H2O2诱导了细胞死亡和缩,而不是其他亡标志物.
联合AM和H2O2治疗补偿了单次治疗中观察到的缺失事件.
抗氧化剂抑制了所有观察到的事件,证实了ROS的参与.

结论:

超氧化物 (O2-) 和过氧化 (H2O2) 协同触发亡及其相关事件.
亚酸和Antimycin A与H2O2的组合可以在诱导亡方面功能性地替代NTAPP激活的溶液.

关键词:

抗菌素A是一种抗菌素.亚斯科布酸是一种亚斯科布酸.细胞死亡细胞死亡 H ((2) O ((2)) 的情况.O(2) (((−) 的意思是这就是ROSOS ROS.这种疾病是spoptosis.

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