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在急性心肌梗塞中,PIWI相互作用的血清RNA的表达和诊断值

Ying Huang¹, Yuan Li², Kaiyu Zhang²

¹Central Laboratory, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, Jiangsu 215008, PR China.

Journal of cardiology

|July 8, 2023

概括

在急性心肌梗塞 (AMI) 患者中,PIWI相互作用的RNAs (piRNAs) 升高. 特定的piRNAs,包括piR-hsa-9010,piR-hsa-28646和piR-hsa-23619,显示出AMI的诊断潜力.

科学领域:

生物化学生物化学
分子生物学分子生物学
心脏病学心脏病学

背景情况:

急性心肌梗塞 (AMI) 是全球主要的死亡原因之一.
识别用于早期诊断和治疗目标的新生物标志物对于改善患者的治疗结果至关重要.

研究的目的:

为了研究PIWI相互作用RNAs (piRNAs) 在AMI患者血清中的表达水平.
探索piRNAs作为AMI的诊断生物标志物和治疗点的潜在作用.

主要方法:

血清RNA从AMI患者和健康对照人群中提取.
使用高通量测序来识别差异表达的piRNAs.
使用定量PCR,ROC曲线分析和KEGG通路分析来验证发现并探索分子机制.

主要成果:

大多数piRNA在AMI患者中都被上调,其中195个被上调和13个被下调.
piR-hsa-9010,piR-hsa-28646和piR-hsa-23619在AMI血清中显著上调,并显示出高诊断价值.
路径分析表明,piR-hsa-23619参与TNF信号路径,piR-hsa-28646参与Wnt信号路径.

结论:

血清piR-hsa-9010,piR-hsa-28646和piR-hsa-23619在AMI中显著上调.
这些piRNAs代表了AMI诊断的有前途的新生物标志物.
它们还可以作为治疗急性心肌梗塞的潜在治疗点.

关键词:

剧烈的心肌梗塞急性心肌梗塞生物标志物生物标志物炎症反应是一种炎症反应.没有信号通路.这就是piRNAs.

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