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伪菌菌E217的高分辨率冷EM结构

Fenglin Li¹, Chun-Feng David Hou¹, Ravi K Lokareddy¹

¹Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA.

Nature communications

|July 8, 2023

概括

这项研究揭示了E217菌体的结构,这对于对抗囊性纤维化相关的Pseudomonas aeruginosa至关重要. 克里奥-EM阐明了它的病毒,尾部机器和基板,识别了宿主O-抗原识别.

科学领域:

结构生物学是结构生物学.
微生物学微生物学
病毒学病毒学

背景情况:

Pseudomonas aeruginosa 是一个重要的机会性病原体,特别是在囊性纤维化患者中.
菌体,如E217,正在被探索为治疗抗生素耐药细菌的治疗剂.
了解菌体结构是优化它们在菌体治疗中的使用的关键.

研究的目的:

为了确定E217菌体病毒的高分辨率结构.
阐明DNA喷射的机制和菌体基板的结构.
为了确定主体受体和尾部纤维相互作用的E217.

主要方法:

低温电子显微镜 (cryo-EM) 被用来解决E217病毒结构.
对19个E217基因产物进行了新的结构确定.
结构分析的重点是尾部基因组喷射机和基板复合体.

主要成果:

确定E217病毒结构的分辨率为3.1 Å (DNA喷射前) 和4.5 Å (DNA喷射后).
解决了66多基板和多个状态的尾部基因组弹射机的完整架构.
发现E217识别了Pseudomonas aeruginosa的O抗原,其尾部纤维相互作用得到了阐明.

结论:

对E217的结构洞察力为其作为对抗Pseudomonas aeruginosa的治疗剂的开发提供了基础.
E217的保存设计原则可能适用于其他类似PB1的Myoviridae菌体.
这项研究促进了对菌体与宿主相互作用和病毒组合的理解.

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