细胞免疫反映了日本COVID-19康复患者中持续存在的症状
Yoshiharu Miyata1, Kohjin Suzuki2, Tatsuya Nagano3
1Division of Bioresource Research and Development, Department of Social/Community Medicine and Health Science, Kobe University Graduate School of Medicine, 1-5-1 Minatojimanakamachi, Chuo-ku, Kobe, 650-0047, Japan.
在解决长期COVID症状方面,T细胞免疫力起着至关重要的作用. 测量COVID-19感染后的T细胞反应可以预测持续的症状或后急性COVID-19综合征 (PACS).
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 病毒学 病毒学
背景情况:
- 在COVID-19后持续的症状,称为长期COVID或后急性COVID-19综合征 (PACS),是一个重大问题.
- 长期COVID的研究主要集中在B细胞免疫力上,T细胞免疫力的作用在很大程度上未被探索.
研究的目的:
- 调查COVID-19康复患者的持续症状数量,细胞因子水平和T细胞免疫反应之间的关系.
- 通过酶相关免疫吸收点 (ELISPOT) 试验评估的T细胞免疫力是否与长期COVID症状的严重程度和持续性相关.
主要方法:
- 来自COVID-19康复患者和健康对照 (HC) 的血样本的回顾性分析.
- 炎症标志物的定量,包括介质白素 (IL) -6,IL-10,IL-18,CXCL9,CCL3和VEGF.
- 使用ELISPOT测试来评估T细胞对SARS-CoV-2抗原 (S1,S2和N蛋白) 的反应.
- 集群分析根据T细胞活性将患者分为高ELISPOT和低ELISPOT组.
主要成果:
- 与HC相比,COVID-19康复患者的IL-6,IL-10,IL-18,CXCL9,CCL3和VEGF水平显著更高.
- 与ELISPOT高组患者相比,ELISPOT低组患者报告的持续症状数量显著增加.
- 这表明T细胞免疫力下降和长期COVID的症状负担增加之间存在相关性.
结论:
- 对于持续的COVID-19症状的快速解决,T细胞免疫非常重要.
- 从COVID-19中恢复后不久的T细胞免疫力的ELISPOT测定测量可以作为长期症状或PACS的预测标记.
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