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基底类和光线A乳腺癌亚型中的DNAJC9表达预测了更差的生存率

Oya Incekara¹, Tolga Acun²

¹Department of Molecular Biology and Genetics, Faculty of Science, Zonguldak Bulent Ecevit University, 67100, Zonguldak, Turkey.

Molecular biology reports

|July 8, 2023

概括

高DNAJC9基因表达与基础性和光线性A乳腺癌的生存率较差有关. 突变和促进物低甲基化不能解释这种增加的表达,这表明DNAJC9是潜在的生物标志物.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
在瘤学瘤学.

背景情况:

乳腺癌涉及遗传和表观遗传的改变.
乳腺癌中DNAJC9基因的预后意义需要进一步调查.

研究的目的:

在乳腺癌中分析DNAJC9的遗传/表观遗传变化.
为了评估DNAJC9在乳腺癌患者的预后价值.

主要方法:

定量逆转录PCR (Q-RT-PCR) 评估了DNAJC9在乳腺细胞系中的表达.
使用bc-GenExMiner与患者数据进行的生存分析.
通过结合双硫酸盐限制分析和UALCAN in-silico工具分析了DNAJC9促进物甲基化.
突变分析采用桑格测序和宇宙数据库.

主要成果:

与正常乳腺组织相比,DNAJC9 mRNA表达在基底类,HER2丰富和光线B亚型中显著升高 (P < 0.001).
在DNAJC9促销器区域中没有发现突变;在临床样本中突变很少 (<1%).
DNAJC9促进子区域在瘤和正常样本中都显示出低甲基化.
增加的DNAJC9表达与基础性和光线性A乳腺癌亚型的不良生存结果相关.

结论:

乳腺癌中高DNAJC9表达不是由突变或促进物低甲基化驱动的.
DNAJC9具有潜在的潜力,作为基础性和光线性A乳腺癌亚型的新生物标志物.

关键词:

生物标志物生物标志物乳腺癌乳腺癌乳腺癌在DNAJC9中.在HSP40中,它是HSP40.

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