化核细胞对地下水化的差异性贡献
Markus Krüger1, Narendrakumar Chaudhari1,2, Bo Thamdrup3
1Aquatic Geomicrobiology, Institute of Biodiversity, Friedrich Schiller University, Jena, Germany.
The ISME journal
|July 8, 2023
概括
完整的氨氧化细菌 (CMX) 在地下水中很丰富,但氨氧化细菌 (AOB) 主要驱动化. 由于高效的生长和酸盐氧化,CMX繁荣发展,保持高种群.
科学领域:
- 环境微生物学环境微生物学
- 生物地质化学循环是什么
- 水生生态系统 水生生态系统
背景情况:
- 完全氧化氨细菌 (CMX) 在地下水中普遍存在,这表明它们在氧化氨细菌 (AOB) 和古生物 (AOA) 上具有竞争优势.
- 在这些环境中,CMX对化过程的确切贡献尚不清楚.
研究的目的:
- 为了区分CMX,AOA和AOB对寡质含水层中化的贡献.
- 为了确定影响这些微生物群体在不同氨和氧水平下利基差异化的环境因素.
主要方法:
- 对CMX氨基单氧化酶亚单元A (amoA) 基因的量化.
- 化率与CMX和AOB类型的相关性分析.
- 使用酸盐和酸盐进行化抑制试验.
- 甲型蛋白质组学分析证实了氧化过程中的活性作用.
- 基于氨和氧需求的生态生理学利基区分的评估.
主要成果:
- 在地下水中检测到的总 amoA 基因中,CMX amoA 基因占了 16-75%.
- 化率与CMX类A和特定的Nitrosomonas ureae (AOB) 类型正相关.
- AOB对氨氧化有显著的贡献,而CMX在氨和酸盐氧化中都很活跃.
- 利基区分与氨和氧的耐受性和代谢多功能性有关.
结论:
- 尽管CMX的数量占据主导地位,但AOB主要控制了寡质地下水中化初始步骤.
- CMX很可能通过较高的生长产量,低氨循环和来自酸盐氧化的能量来维持高种群.
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