相关实验视频

Updated: Jul 24, 2025

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing ChIP-seq

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing ChIP-seq

Published on: January 18, 2020

在Chaetomium thermophilum中糖调节促进体的识别和表征

Sven Reislöhner¹, Geza Schermann², Max Kilian³

¹Biochemistry Center (BZH), University of Heidelberg, Im Neuenheimer Feld 328, 69120, Heidelberg, Germany.

BMC biotechnology

|July 8, 2023

概括

研究人员在热友真菌Chaetomium thermophilum中发现了新型的酶可调节的促进物. 这些促进体使糖控制基因表达成为可能,促进了这一重要的真核模型生物体的功能研究.

科学领域:

分子生物学分子生物学
菌类学菌类学是指菌类学.
生物化学生物化学

背景情况:

热菌 (Chaetomium thermophilum) 是一种热爱菌类,对于蛋白质复合物的结构和生化研究至关重要.
由于缺乏兼容的遗传工具,C. thermophilum的功能分析一直受到限制,与Saccharomyces cerevisiae.等中性模型不同.

研究的目的:

在C. thermophilum中识别糖调节的基因表达系统.
在这种热爱真菌中进行功能遗传研究的新型促进者的特征.

主要方法:

在西洛斯与葡萄糖条件下对基因表达的比较分析.
在黄色光蛋白 (YFP) 记者基因上游的确定的促进体 (XYL和XDH) 的克隆.
使用西式涂抹和活细胞光显微镜验证促进剂活性.

主要成果:

鉴定了基因,其表达由克西洛斯诱导,并被葡萄糖抑制.
由克隆的XYL和XDH促进体驱动的表达了依赖于基的YFP表达.
成功诱导特定的核糖体组装缺陷使用XDH促进剂在含有西洛斯的介质.

结论:

在C. thermophilum中发现了可调节酶的新型促进体.
这些促进体为未来热友性真核生物的功能遗传研究提供了宝贵的工具.
开发的遗传工具增强了C. thermophilum作为模型生物的实用性.

关键词:

核生物热爱动物.状真菌是一种状真菌.可以诱导的促进者.热稳定蛋白质是一种热稳定蛋白质.转录组转录组是一个转录组.

更多相关视频

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

相关概念视频

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

The Eukaryotic Promoter Region

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...