相关实验视频

Updated: Jul 24, 2025

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

牙芽细胞释放外体,以调节牙芽细胞干细胞的牙芽细胞分化

Xinghong Luo¹, Weiqing Feng², Shijiang Huang³

¹Department of Endodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, No 366 Jiangnan Avenue South, Guangzhou, 510280, Guangdong, China.

Stem cell research & therapy

|July 8, 2023

概括

拉巴胺素复合体1 (mTORC1) 激活的机械标在牙细胞中调节了外体释放,抑制了牙纸干细胞的分化. 这一发现为牙 - 纸复合物的再生和牙生成提供了新的见解.

科学领域:

*再生医学是一种再生医学.
* * 干细胞生物学
* 分子生物学 * 分子生物学

背景情况:

* 牙髓干细胞 (DPSC) 对于牙-牙髓复合体的再生至关重要.
*了解DPSC静止机制是改善牙生成的关键.
* 牙细胞在牙形成中起着至关重要的作用.

研究的目的:

* 调查拉巴胺素复合体1 (mTORC1) 的机械点在调节DPSC行为的作用.
* 探索mTORC1活动在牙质细胞对牙形成和DPSC分化的影响.
* 阐明由芽细胞衍生的外体对DPSC分化产生影响的机制.

主要方法:

*生成TSC1条件淘汰赛 (CKO) 鼠标来调节mTORC1活动.
*采用MDPC23细胞 (质细胞类细胞) 和DPSCs进行体外研究,包括共培和外体组分析.
*使用的技术:H&E染色,免疫光,微型CT,传输电子显微镜,纳米粒子跟踪分析,阿利扎林红色S染色,ALP染色,qRT-PCR,西部涂抹和miRNA测序.

主要成果:

*mTORC1激活在牙细胞中导致牙更厚,牙体积增加.
* 胞细胞外体释放与mTORC1活性有负相关性.
*不管mTORC1的活性如何,来自体细胞的外体细胞以度依赖的方式抑制了DPSC的体细胞分化,而不会改变外体小RNA含量.

结论:

*mTORC1信号在口腔细胞中控制着外体细胞的释放,这反过来又抑制了DPSC的分化.
* 外体含量,特别是miRNAs,没有受到mTORC1活动的改变.
*这些发现为牙-肉复合体再生过程的调节提供了新的视角.

关键词:

提供服务的DPSCs.外基因组是外基因组的组成部分.形体的分化形体分化它们是Odontoblasts.在 mTORC1 的情况下,mTORC1 是 mTORC1.

更多相关视频

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Bone Cells and Tissue

Bone Cells and Tissue

Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...