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相关概念视频

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

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相关实验视频

Updated: Jun 27, 2026

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
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单个细胞外VEsicle纳米显微镜

Andras Saftics1, Sarah Abuelreich1, Eugenia Romano1

  • 1Department of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.

Journal of extracellular vesicles
|July 8, 2023
PubMed
概括

我们开发了一种新的测定方法,单细胞外囊泡纳米镜 (SEVEN),以精确分析细胞外囊泡 (EV) 亚群. SEVEN可以在胰腺癌患者中识别独特的EV标志物,为新的诊断工具提供潜力.

关键词:
七个 七个 七个细胞外囊泡 (EVs) 的产生.纳米显微镜的使用方法胰腺管腺癌 (PDAC) 是一种定量单分子定位显微镜 (qSMLM)单一的EV分析

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科学领域:

  • 生物技术是生物技术.
  • 纳米技术 纳米技术
  • 生物标志物发现发现

背景情况:

  • 细胞外囊泡 (EVs) 是有前途的生物标志物,但对其子群的隔离和表征仍然具有挑战性.
  • EVs是由四素 (CD9,CD63,CD81) 和源特定标记物定义的.
  • 需要强大的方法来进行全面的EV亚群评估.

研究的目的:

  • 开发和验证一种新的试验,以全面描述EV亚群的特征.
  • 评估该试验在识别与疾病相关的EV变化的有用性.
  • 探索电动汽车作为胰腺癌生物标志物的潜力.

主要方法:

  • 结合亲和度隔离与超分辨率成像用于EV分析.
  • 开发了单细胞外膀纳米扫描 (SEVEN) 试验.
  • 量化EV数,大小,形状和分子含量 (四素).

主要成果:

  • SEVEN试验成功量化了来自人体血的EV亚群.
  • 检测到EV数量和样本稀释之间的正相关性.
  • 七个检测到EVs从最小的样本体积 (∼0.1μL).
  • 具有CD9,CD63和CD81丰富的EV的特征大小,形状和四氨酸含量.
  • 在胰腺癌患者中确定了不同的EV亚群 (例如,较小的CD9丰富EV,较大的IGF1R丰富EV).

结论:

  • SEVEN试验提供了一种经过验证的方法,用于详细描述EV亚群的特征.
  • SEVEN展示了识别与胰腺癌等疾病相关的独特EV签名的潜力.
  • 这个平台可以用于描述与疾病相关的和与器官相关的EV亚群.