近紫外线照射刺激未经修改的聚乙烯基,以实现稳定和可持续的抗菌活性
Chongxing Liu1, Zhuo Huang2, Jinlei Zhu1
1School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325000, China.
Colloids and surfaces. B, Biointerfaces
|July 9, 2023
概括
未经修改的聚乙基 (PEEK) 在近紫外线下表现出可持续的抗菌活性,为牙科应用提供了新的解决方案. 这种PEEK材料表现出良好的细胞兼容性和稳定的抗微生物特性,为增强的牙科修复铺平了道路.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 摄影化学的使用.
背景情况:
- 聚乙基 (PEEK) 是一种广泛使用的生物材料,特别是在牙科中,因为它具有出色的机械性能.
- 然而,PEEK缺乏固有的抗菌特性,这可能是预防手术后感染的限制.
- 研究如何向PEEK赋予可持续的抗菌作用的方法,对于提高其临床疗效至关重要.
研究的目的:
- 评估未经修改的PEEK在暴露于365nm近紫外线 (NUV) 光时的细胞毒性和可持续的抗菌疗效.
- 在NUV光照射下初步阐明PEEK的抗菌机制.
- 评估NUV处理的PEEK在牙科应用中的潜力.
主要方法:
- PEEK样品经过NUV光处理 (365nm,5W,30分钟).
- 使用MC3TC-E1细胞评估了细胞毒性.
- 使用殖民地形成单元 (CFU) 试验和扫描电子显微镜 (SEM) 评估了对常见口腔细菌的抗菌活性.
- 通过光谱测量和乳酸脱酶试验探索了抗菌机制.
- 进行循环抗菌试验和测量水接触角,以评估可持续性和表面性能.
主要成果:
- 在PEEK中,没有显著的细胞毒性 (P > 0.05).
- 对黄金葡萄球菌,突变葡萄球菌,高尔多尼葡萄球菌和血球葡萄球菌观察到显著的抗菌作用,但不是对大肠杆菌 (P < 0.05).
- SEM证实了细菌抑制,光谱测量表明存在单片氧.
- 乳酸脱酶测定验证了Staphylococcus aureus中的膜破裂.
- 表面特性保持稳定,循环测试证实了可持续的抗菌活性.
结论:
- 未经修改的PEEK在NUV光处理 (365nm) 下表现出稳定和可持续的抗菌特性,具有良好的细胞相容性.
- 这种方法提供了一个新的策略,以克服PEEK的非抗菌性质.
- 这些发现为PEEK在牙科的扩大应用提供了理论基础.
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