人类胎盘中的SARS-CoV-2位由空间转录组学揭示出来
Enrico R Barrozo1, Maxim D Seferovic1, Eumenia C C Castro2
1Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX, USA.
Med (New York, N.Y.)
|July 9, 2023
概括
高分辨率的胎盘转录学揭示了SARS-CoV-2感染如何影响特定的胎盘. 病毒载量高的位显示出炎症和免疫反应的改变,无论母亲的症状如何.
科学领域:
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 胎盘对于分离母亲和胎儿抗原以及防止病原体传播至关重要.
- 了解胎盘转录档案可以识别专门的微环境及其功能.
研究的目的:
- 为了创建一个高分辨率的胎盘转录的地图.
- 研究胎盘微环境的功能和转录特征.
- 为了检查SARS-CoV-2感染对胎盘的影响.
主要方法:
- 使用Visium空间转录组生成了17927个空间转录组.
- 与273,944个胎盘单细胞和单核转录组集成.
- 从健康对照组和COVID-19感染参与者的胎盘分析 (无症状和有症状).
主要成果:
- 形成了至少有22个子群的胎盘图谱.
- 在症状和无症状的病例中,检测到SARS-CoV-2在同胞性热细胞中.
- 有高SARS-CoV-2水平的胎盘处表现出促炎反应,改变金属酶信号和免疫细胞转移.
- 基因表达反应的胎儿性别差异有限,主要观察到男性的母性决定性.
结论:
- 高分辨率的空间转录学揭示了胎盘对SARS-CoV-2的动态反应.
- 这些反应发生在协调的微环境中,无论疾病的临床严重程度如何.
- 这项研究直接证实了胎盘内的功能性微环境.
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