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使用金属有机框架作为信号标签的重组糖蛋白的无生物识别元素的电化学检测

Yong Chang¹, Gang Liu², Shuang Li³

¹Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, 455000, People's Republic of China.

Analytica chimica acta

|July 9, 2023

概括

一种新的抗体和无酶的电化学方法使用顺序化学识别检测重组糖蛋白. 这种方法为生物制药研究,反兴奋剂和临床诊断提供了一种敏感且具有成本效益的工具.

科学领域:

生物化学生物化学
分析化学分析化学
材料科学材料科学材料科学

背景情况:

准确检测重组糖蛋白对于贫血治疗和反兴奋剂工作至关重要.
现有的方法通常依赖于抗体或酶,这可能是昂贵和复杂的.
存在对敏感,高效和可访问的糖蛋白检测技术的需求.

研究的目的:

开发一种无抗体和无酶的电化学方法,用于检测重组糖蛋白.
使用顺序化学识别对六西丁 (His₆) 标签和甘氨酸残留物.
为生物制药研究,反兴奋剂和临床诊断建立一个敏感和可靠的方法.

主要方法:

用修改了酸-Ni2+复合体 (MBs-NTA-Ni2+) 的磁珠被用于捕获His6标记的糖蛋白.
用酸修饰的铜基金属有机框架 (Cu-MOFs) 用于与糖残留物结合.
电化学信号是由MOF中的Cu2+离子生成的,用于放大检测.

主要成果:

该方法显示了高灵敏度和广泛的线性检测范围 (0.0150 ng/mL) 复合人体红色素.
达到了5.3 pg/mL的低检测极限.
这种方法在敏感和选择性糖蛋白确定方面被证明是有效的.

结论:

拟议的电化学方法提供了一种简单,具有成本效益和敏感的方法来检测重组糖蛋白.
这种技术对生物制药研究,反兴奋剂分析和临床诊断的应用具有重大前景.
序列化学识别策略为糖蛋白分析提供了一个强大的平台.

关键词:

酸酸是一种酸.电化学生物传感器黑色胺标签的标签金属有机框架的框架.再组合型葡萄糖蛋白质是什么

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