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相关概念视频

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
439

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相关实验视频

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High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings
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使用光学捕获的单个向上转换发光的细胞温度探测.

K Suresh1, K Monisha1, Aseefhali Bankapur1

  • 1Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, 576104, India.

Analytica chimica acta
|July 9, 2023
PubMed
概括

这项研究研究了用于纳米尺度温度探测的升级纳米粒子 (UCNPs) 的温度灵敏度. 裸体UCNP显示出比涂有或涂有金的UCNP更高的热敏度,特别是在生物细胞内.

关键词:
细胞温度探测 探测细胞温度发光的光度是非常的低.光学陷的捕捉方式等离子体纳米粒子的使用.热敏度 热敏度 热敏度 热敏度上升转换是一种上升转换.

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相关实验视频

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科学领域:

  • 纳米技术纳米技术
  • 材料科学 材料科学 材料科学
  • 生物物理学的生物物理.

背景情况:

  • 稀土元素化纳米粒子 (UCNPs) 的上转换发光被研究用于纳米级温度探测.
  • 低量子效率和表面修改对温度灵敏性的作用仍然是挑战.
  • 调查表面被动化和等离子粒子对UCNP单颗粒子温度敏感性的影响至关重要.

研究的目的:

  • 在单个粒子层面研究裸体UCNP,UCNP@SiO2和UCNP@SiO2@Au粒子的温度灵敏度.
  • 探索表面被动化 (外) 和等离子粒子结合 (黄金) 对UCNP热灵敏度的影响.
  • 评估使用光学捕获的UCNP用于细胞内温度测量的可行性.

主要方法:

  • 在一个生理温度范围内 (299 K319 K) 光学捕捉单个UCNP (裸体,UCNP@SiO2,UCNP@SiO2@Au).
  • 从热合的能量状态测量发光,以确定热灵敏度.
  • 在生物细胞内使用光学捕获的UCNP进行现场温度测量.

主要成果:

  • 裸体UCNP表现出比水性介质中的UCNP@SiO2和UCNP@SiO2@Au更高的热相对敏感性.
  • 在细胞内被光学捕获的UCNP表现出对温度的绝对敏感性增加.
  • 与生物细胞环境中的修饰UCNP相比,裸体UCNP显示出更高的热敏感性.

结论:

  • 使用光学捕获的UCNP可以实现单颗粒水平温度测量.
  • 表面被动化和等离子体颗粒的结合可以降低UCNP的热灵敏度.
  • UCNP的热灵敏度受到周围环境的影响,这凸显了生物系统中现场测量的重要性.