机械敏感的引力产生是由中性粒细胞激活状态调节的
Hadley Witt1,2, Zicheng Yan3, David Henann3
1Graduate Program in Pathobiology, Brown University, Providence, RI, 02912, USA. hadley_witt@brown.edu.
Scientific reports
|July 9, 2023
概括
中性粒细胞激活显著影响力产生,而败血症患者表现出增加的力. 一种新的2D成像方法允许对这些力量进行详细分析,进步我们对中性粒细胞在宿主防御中的功能的理解.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 中性粒细胞是关键的免疫细胞,它们的引力对宿主防御功能,如迁移和细胞分裂至关重要.
- 了解中性粒细胞激活如何影响力产生至关重要,但在实验上具有挑战性.
- 上一篇 引力显微镜 (TFM) 方法需要复杂的3D成像来准确测量力.
研究的目的:
- 为了研究中性粒细胞激活对引力产生的影响.
- 应用一种新的基于二维成像的TFM技术来分析细胞力量.
- 为了比较来自败血症患者的中性粒细胞与健康捐赠者的力量生成.
主要方法:
- 开发了一种新的2D成像模式,用于捕捉中性粒细胞中的外平面力.
- 使用基于拓的单粒子跟踪算法和有限元素方法计算.
- 应用了光显微镜来量化平面内和平面外的引力.
主要成果:
- 来自败血症患者的中性粒细胞产生了比健康捐赠者的总力量大得多.
- 在败血性中性粒细胞中,大多数增加的力被观察到与基质的平面内.
- 健康中性粒细胞的ex vivo激活显示出多种效应,包括某些刺激的强度下降.
结论:
- 这种基于光的新型TFM方法可以对中性粒细胞的引力进行详细分析.
- 中性粒细胞的激活,特别是在败血症中,导致失调的力量产生.
- 这项技术有助于在生物学上有意义的环境中研究中性粒细胞机制.
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