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循环RNA PGPEP1诱导结直肠癌恶性病变和免疫逃生

Chi Zhang¹, ChengZhao Zhang², XinLu Liu²

¹Department of Gastrointestinal Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.

Cell cycle (Georgetown, Tex.)

|July 10, 2023

概括

循环RNAcircPGPEP1通过调节miR-515-5p/NFAT5轴促进结直肠癌 (CRC) 的进展和免疫逃生. 这项研究揭示了circPGPEP1作为CRC治疗的潜在治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

结肠直肠癌 (CRC) 是一个主要的全球健康问题.
循环RNAs (circRNAs) 参与CRC病变发生.
在CRC恶性瘤和免疫逃避中hsa_circ_0050102 (circPGPEP1) 的特定作用在很大程度上是未知的.

研究的目的:

为了研究circPGPEP1在结直肠癌中的功能.
阐明circPGPEP1在CRC进展和免疫逃逸中的调节机制.
探索circPGPEP1/miR-515-5p/NFAT5轴作为CRC中的治疗点的潜力.

主要方法:

使用生物信息学分析和circRNA免疫沉降试验.
路西法酶记者测定,RIP,RNA下拉,和FISH证实了分子相互作用.
共同培养试验,CFSE试验和流细胞测量评估了在抗瘤免疫力中的功能性作用.

主要成果:

发现circPGPEP1在CRC组织中表达高且稳定.
沉默circPGPEP1抑制了CRC细胞的增殖,迁移,上皮细胞-介质细胞过渡 (EMT) 和免疫逃逸,同时在体外促进了细胞亡.
在体内,circPGPEP1促进了CRC瘤的生长和免疫逃脱.
circPGPEP1通过海绵化miR-515-5p作用,对激活T细胞5 (NFAT5) 表达的核因子进行上调.

结论:

在结直肠癌中,circPGPEP1作为瘤基因起作用.
环PGPEP1/miR-515-5p/NFAT5轴在CRC进展和免疫逃避中发挥着至关重要的作用.
circPGPEP1代表了结直肠癌的潜在治疗标.

关键词:

循环RNA PGPEP1 的情况.结肠直肠癌是什么意思免疫系统逃脱,逃离免疫系统.微型RNA-515-5p是什么意思激活T细胞5的核因子

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